CAJ | 학술논문

目的：探讨肿瘤增殖型腺病毒Ad-delE1b55KD-shRNA/survivin-EGFP介导的针对survivin的高选择性RNA干扰对在体结肠癌细胞 HT-29增殖和凋亡的影响。方法用携带针对人survivin的shRNA的肿瘤增殖型腺病毒 Ad-delE1b55KD-shRNA/survivin-EGFP转染结肠癌细胞HT-29裸鼠移植瘤模型，以携带相同shRNA的复制缺陷型腺病毒和脂质体载体为对照，检测移植瘤体变化，以及 TUNEL 法检测肿瘤细胞凋亡率。结果 Ad-delE1b55KD-shRNA/survivin-EGFP 介导的高选择性RN A干扰使结肠癌裸鼠移植瘤的瘤体减小、移植瘤细胞凋亡率显著增加，且高于目前常用的复制缺陷型腺病毒载体和脂质体载体（P＜0．05）。结论 Ad-delE1b55KD-shRNA/survivin-EGFP介导的高选择性RNA干扰能高效地诱导结肠癌细胞凋亡并抑制其增殖。
목적：탐토종류증식형선병독Ad-delE1b55KD-shRNA/survivin-EGFP개도적침대survivin적고선택성RNA간우대재체결장암세포 HT-29증식화조망적영향。방법용휴대침대인survivin적shRNA적종류증식형선병독 Ad-delE1b55KD-shRNA/survivin-EGFP전염결장암세포HT-29라서이식류모형，이휴대상동shRNA적복제결함형선병독화지질체재체위대조，검측이식류체변화，이급 TUNEL 법검측종류세포조망솔。결과 Ad-delE1b55KD-shRNA/survivin-EGFP 개도적고선택성RN A간우사결장암라서이식류적류체감소、이식류세포조망솔현저증가，차고우목전상용적복제결함형선병독재체화지질체재체（P＜0．05）。결론 Ad-delE1b55KD-shRNA/survivin-EGFP개도적고선택성RNA간우능고효지유도결장암세포조망병억제기증식。
Objective To investigate the effect of RNAi-mediated survivin gene with conditionally replicating adenovirus Ad-de-lE1b55KD-shRNA/survivin-EGFP silencing on propagation and apoptosis of colon carcinoma cell lines HT-29 .Methods Ad-de-lE1b55KD-shRNA/survivin-EGFP was transfected to transplantation tumor on athymic mouse ,replication defective adenovirus and liposome vector which was contained the same shRNA as Ad-delE1b55KD-shRNA/survivin-EGFP were as control one .Then the change of transplantation tumor on athymic mouse was tested ,and TUNEL was used to assay the apoptosis rate of transplantation tumor cells .Results After transfection of Ad-delE1b55KD-shRNA/survivin-EGFP ,the quality of transplantation tumor on athymic mouse were reduced and the apoptosis rate of transplantation tumor cells were higher than replication defective adenovirus and lipo -some vector which was contained the same shRNA (P<0 .05) .Conclusion The effect of Ad-delE1b55KD-shRNA/survivin-EGFP to colon carcinoma cell on propagation and apoptosis were higher than replication defective adenovirus and liposome vector .