CAJ | 학술논문

目的：探讨Akt信号通路在右美托咪定预处理对抗脂多糖（LPS）致急性肺损伤（ALI）中的作用。方法成年SD大鼠24只，随机分为对照组（T0组）、LPS注射后1 h（T1组）、3 h（T2组）和6 h组（T3组），检测肺组织中Akt和p-Akt的含量。大鼠54只随机分为对照组（C组）、ALI 6 h组（ALI组）和右美托咪定＋ ALI 6 h组（D＋ ALI组），检测 p-Akt表达、肺泡灌洗液（BALF）蛋白浓度、细胞凋亡指数，观察肺组织病理变化。大鼠40只随机分为ALI组和D＋ALI组，观察48 h生存率。结果与T0组比较，T1、T2和T3组p-Akt表达依次减少（P＜0．05）。与ALI组比较，D＋ALI组p-Akt表达明显升高（P＜0．05），但仍低于C组（P＜0．05），BALF蛋白浓度，细胞凋亡指数均降低（P＜0．05），但仍高于C组（P＜0．05）；D＋ ALI组病理损伤较ALI组减轻。D＋ALI组48 h生存率明显高于ALI组（P＜0．05）。结论右美托咪定可能通过增加ALI组织中p-Akt的浓度发挥保护作用。
목적：탐토Akt신호통로재우미탁미정예처리대항지다당（LPS）치급성폐손상（ALI）중적작용。방법성년SD대서24지，수궤분위대조조（T0조）、LPS주사후1 h（T1조）、3 h（T2조）화6 h조（T3조），검측폐조직중Akt화p-Akt적함량。대서54지수궤분위대조조（C조）、ALI 6 h조（ALI조）화우미탁미정＋ ALI 6 h조（D＋ ALI조），검측 p-Akt표체、폐포관세액（BALF）단백농도、세포조망지수，관찰폐조직병리변화。대서40지수궤분위ALI조화D＋ALI조，관찰48 h생존솔。결과여T0조비교，T1、T2화T3조p-Akt표체의차감소（P＜0．05）。여ALI조비교，D＋ALI조p-Akt표체명현승고（P＜0．05），단잉저우C조（P＜0．05），BALF단백농도，세포조망지수균강저（P＜0．05），단잉고우C조（P＜0．05）；D＋ ALI조병리손상교ALI조감경。D＋ALI조48 h생존솔명현고우ALI조（P＜0．05）。결론우미탁미정가능통과증가ALI조직중p-Akt적농도발휘보호작용。
Objective To investigate the role of Akt signaling in the protective effect of dexmedetomidine preconditioning on the lipopolysaccharide(LPS)-induced acute lung injury (ALI) in rat .Methods Twenty-two SD rats were randomly divided into 4 groups:before LPS injection group(T0 group) ,1 h after LPS injection group(T1 group) ,3 h after LPS injection group(T2 group) and 6 h after LPS injection group(T3 group) ,the expression of Akt and p-Akt in the process of LPS-induced ALI was investigated . Another 54 rats were divided into 3 groups :control group(C group) ,6 hours of ALI group(ALI group) and Dexmedetomidine+ALI group(D+ ALI group) ,the expression of p-Akt in lung tissues ,the protein concentration in bronchoalveolar lavage fluid (BALF) ,apoptotic index and the pathological changes in the lung tissues were observed .Forty rats were divided into ALI group and ALI + D group to investigate the 48 h-survival ratio .Results Compared with T0 group ,the level of p-Akt expression in T1 ,T2 , T3 groups were deceased in a time-dependent manner(P<0 .05) .Compared with ALI group ,p-Akt in D + ALI group increased significantly(P<0 .05) but was still lower than that in C group(P<0 .05);the protein concentration in BALF and the apoptotic in-dex in D + ALI groups were significantly lower than those in ALI group(P<0 .05) ,but still higher than those in C group(P<0 .05) .The 48 h survival ratio was significantly decreased in D + ALI group comparing with ALI group(P<0 .05) .Conclusion Dexmedetomidine preconditioning might display protective effect via activating p-Akt signaling pathway in LPS-induced ALI .