重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2013年
28期
3398-3399,3402
,共3页
MIP-1α%B7-1%乳腺肿瘤%免疫应答
MIP-1α%B7-1%乳腺腫瘤%免疫應答
MIP-1α%B7-1%유선종류%면역응답
MIP-1α%B7-1%breast neoplasms%immune response
目的：探讨共染MIP-lα与B7-1诱导乳腺癌大鼠的免疫应答能力。方法采用SHZ-88乳腺癌细胞造模；每日观察SD大鼠接种乳腺癌细胞后有无肿瘤生长；将荷瘤大鼠随机分成对照组、M IP-1α组、B7-1组和联合组注射对应的转基因细胞进行治疗，分别在1～4周观察肿瘤体积变化和生存时间，收集大鼠外周血检测CD4＋、CD8＋百分比和CD4＋/CD8＋比值以及白细胞介素2（IL-2）、人γ干扰素（IFN-γ）水平并进行比较。结果对 SD大鼠分别接种SHZ-88、SHZ-88/MIP-1α、SHZ-88/B7-1及SHZ-88/MIP-1α＋B7-1细胞10 d后，各组的致瘤率分别为100％、20％、30％及0％；对照组、MIP-1α组、B7-1组和联合组注射对应的转基因细胞治疗后，联合组的肿瘤体积在1～4周均明显小于其他组，生存时间明显长于其他组，外周血CD4＋、CD8＋百分比和CD4＋/CD8＋比值以及IL-2、IFN-γ水平均明显高于其他组，以上比较差异均有统计学意义（ P＜0．05）。结论共染M IP-lα与B7-1，可诱导乳腺癌大鼠机体的免疫应答能力，起到抗肿瘤的作用。
目的：探討共染MIP-lα與B7-1誘導乳腺癌大鼠的免疫應答能力。方法採用SHZ-88乳腺癌細胞造模；每日觀察SD大鼠接種乳腺癌細胞後有無腫瘤生長；將荷瘤大鼠隨機分成對照組、M IP-1α組、B7-1組和聯閤組註射對應的轉基因細胞進行治療，分彆在1～4週觀察腫瘤體積變化和生存時間，收集大鼠外週血檢測CD4＋、CD8＋百分比和CD4＋/CD8＋比值以及白細胞介素2（IL-2）、人γ榦擾素（IFN-γ）水平併進行比較。結果對 SD大鼠分彆接種SHZ-88、SHZ-88/MIP-1α、SHZ-88/B7-1及SHZ-88/MIP-1α＋B7-1細胞10 d後，各組的緻瘤率分彆為100％、20％、30％及0％；對照組、MIP-1α組、B7-1組和聯閤組註射對應的轉基因細胞治療後，聯閤組的腫瘤體積在1～4週均明顯小于其他組，生存時間明顯長于其他組，外週血CD4＋、CD8＋百分比和CD4＋/CD8＋比值以及IL-2、IFN-γ水平均明顯高于其他組，以上比較差異均有統計學意義（ P＜0．05）。結論共染M IP-lα與B7-1，可誘導乳腺癌大鼠機體的免疫應答能力，起到抗腫瘤的作用。
목적：탐토공염MIP-lα여B7-1유도유선암대서적면역응답능력。방법채용SHZ-88유선암세포조모；매일관찰SD대서접충유선암세포후유무종류생장；장하류대서수궤분성대조조、M IP-1α조、B7-1조화연합조주사대응적전기인세포진행치료，분별재1～4주관찰종류체적변화화생존시간，수집대서외주혈검측CD4＋、CD8＋백분비화CD4＋/CD8＋비치이급백세포개소2（IL-2）、인γ간우소（IFN-γ）수평병진행비교。결과대 SD대서분별접충SHZ-88、SHZ-88/MIP-1α、SHZ-88/B7-1급SHZ-88/MIP-1α＋B7-1세포10 d후，각조적치류솔분별위100％、20％、30％급0％；대조조、MIP-1α조、B7-1조화연합조주사대응적전기인세포치료후，연합조적종류체적재1～4주균명현소우기타조，생존시간명현장우기타조，외주혈CD4＋、CD8＋백분비화CD4＋/CD8＋비치이급IL-2、IFN-γ수평균명현고우기타조，이상비교차이균유통계학의의（ P＜0．05）。결론공염M IP-lα여B7-1，가유도유선암대서궤체적면역응답능력，기도항종류적작용。
Objective To investigate the ability of immune response of cotransfection of rat breast cancer cells with MIP-1αand B7-1 .Methods Tumor growth was observed every day after the SHZ-88 breast cancer cells were inoculated in the rats .The tumor-bearing rats were randomly divided into control group ,MIP-1αgroup ,B7-1 group and combination group ,which were injected with corresponding transgenic cells .The change of tumor volume in 1~4 weeks and the survival time were observed and compared .The peripheral blood was collected .CD4+ ,CD8+ percentage and CD4+ /CD8+ ratio and the levels of IL-2 and IFN-γof the peripheral blood were detected and compared .Results The tumorigenic rate were 100% ,20% ,30% and 0% after the SD rats were inoculated with SHZ-88 ,SHZ-88/MIP-1α,SHZ-88/B7-1 and SHZ-88/MIP-1α+B7-1 cells for 10 days .After the transgenic therapy ,the tumor volume of the combination group in 1~4 weeks were significantly smaller than the other groups .The survival time of the combina-tion group was significantly longer than the other groups .The CD4+ ,CD8+ percentage and CD4+ /CD8+ ratio and the levels of IL-2 and IFN-γof peripheral blood in the combination group were significantly higher than the other groups .The differences above were all statistically significant (P<0 .05) .Conclusion Cotransfection of MIP-1αand B7-1 could induce immune response in breast cancer rats and play anti-cancer effects .
