CAJ | 학술논문

目的：观察中药“复方鳖甲软肝片方”对TGF-β1诱导的肾间质成纤维细胞(NRK-49F)增殖及分泌细胞外基质的影响。方法：以2 ng/ml hTGF -β1诱导 NRK -49F 细胞，并以软肝片方大、中、小剂量(分别以7g/kg、3.5 g/kg、1.75 g/kg)药物血清进行干预，分别应用MTT法、ELISA、免疫细胞化学方法，观察肾间质成纤维细胞增殖、细胞上清FN浓度及ColⅠ、ColⅢ的表达。结果：软肝方含药血清对hTGF-β1诱导的细胞增殖无影响；但大、中剂量软肝方能不同程度地降低FN的分泌及ColⅠ、ColⅢ的表达，软肝方大剂量组作用最强(P<0.01)。结论：复方鳖甲软肝片方在一定程度上抑制TGF-β1诱导的肾间质成纤维细胞ColⅠ或ColⅢ的表达，大剂量软肝方作用最强。
목적：관찰중약“복방별갑연간편방”대TGF-β1유도적신간질성섬유세포(NRK-49F)증식급분비세포외기질적영향。방법：이2 ng/ml hTGF -β1유도 NRK -49F 세포，병이연간편방대、중、소제량(분별이7g/kg、3.5 g/kg、1.75 g/kg)약물혈청진행간예，분별응용MTT법、ELISA、면역세포화학방법，관찰신간질성섬유세포증식、세포상청FN농도급ColⅠ、ColⅢ적표체。결과：연간방함약혈청대hTGF-β1유도적세포증식무영향；단대、중제량연간방능불동정도지강저FN적분비급ColⅠ、ColⅢ적표체，연간방대제량조작용최강(P<0.01)。결론：복방별갑연간편방재일정정도상억제TGF-β1유도적신간질성섬유세포ColⅠ혹ColⅢ적표체，대제량연간방작용최강。
Objective:Objective:in vitro,we object the effect of Compound Biejia Ruangan Precription( CBRP) on renal in-terstitial fibroblast proliferation and excreting extracellular matrix induced by TGF-β1 . Methods:Using method of serological pharma-cology, rat renal interstitial fibroblast (NRK-49F cell) stimulated with TGF-β1, simultaneously 10% different rat serum was add-ed to for 48 h as follows:the normal rat serum( male SD rats were given physiological saline by gastric gavages) , the low dose CBRP-medicated rat serum (given CBRP 7 g/kg), the middling dose one(given CBRP 3. 5 g/kg), the high dose one(given CBRP 1. 75 g/kg), applying to immunocytochemistry, MTT assay, ELISA, cell proliferation and extracellular matrix such as fibronectin, typeⅠcollagen, typeⅢ collagen were detected. Results:Contrasted with the normal rat serum: rat serum medicated-CBRP had no effect on NRK-49F cell proliferation which induced by TGF-β1 , but in the groups of rat serum medicated- high dose CBRP, the high level of cell secretion fibronectin, collagenⅠor collagenⅢwhich boosted by TGF-β1 had been decreased, especially the group of high dose CBRP had best effect (P<0. 01). Conclusion:In some extent CBRP could restrain renal interstitial fibroblast secrete or synthesize extracellular matrix such as FN, Col, ColⅢwhich enhanced by TGF-β1 , especially the high dose CBRP exert preferable effects.