CAJ | 학술논문

目的：研究核糖体蛋白S15a（ribosomal protein S15a RPS15a）基因在胃癌及癌旁组织中表达差异，为进一步了解胃癌发生、发展的分子机制提供帮助。方法应用荧光定量PCR等方法进一步验证该基因在胃癌及癌旁组织中的表达差异，并在多种肿瘤细胞中的表达量进行比较。结果该基因在胃癌组织中的表达量高于其对应的癌旁组织，但在多种肿瘤细胞中的表达量无显著差异。结论 RPS15a基因在胃癌中呈高表达，说明其可能与细胞恶性生物学行为相关。在多种肿瘤细胞中表达量无显著差异，推测其可能在恶性肿瘤中的高表达具有普遍性。
목적：연구핵당체단백S15a（ribosomal protein S15a RPS15a）기인재위암급암방조직중표체차이，위진일보료해위암발생、발전적분자궤제제공방조。방법응용형광정량PCR등방법진일보험증해기인재위암급암방조직중적표체차이，병재다충종류세포중적표체량진행비교。결과해기인재위암조직중적표체량고우기대응적암방조직，단재다충종류세포중적표체량무현저차이。결론 RPS15a기인재위암중정고표체，설명기가능여세포악성생물학행위상관。재다충종류세포중표체량무현저차이，추측기가능재악성종류중적고표체구유보편성。
Objective To explore the difference of the expression of ribosomal protein S15a RPS15a is the gastric carcinoma and paracancerous tissue, for better understanding the molecular mechanism of the carcinogenesis and progress of gastric carcinoma. Method Fluorescent quantitive PCR was applied to test the difference of the gene expression in the gastric carcinoma and paracancerous tissue, which were compared with the its expression in the various types to tumor cells. Result RPS15a was highly expressed in the gastric carcinoma, indicating its relationship with the malignant biological hehavior of the tumor cells. The espressions were not significantly different among various types of tumor cells, which implies than such expression may be universal in malignant tumors.