CAJ | 학술논문

利用台盼蓝排染法检测Leukamenin E对HL-60细胞生长的抑制作用；在倒置显微镜及荧光显微镜下分别检测细胞和细胞核形态变化；进一步采用流式细胞术和AO/EB荧光双染法检测Leukamenin E对 HL-60细胞周期分布的影响及诱导细胞凋亡的作用．结果表明，化合物Leukamenin E对HL-60细胞生长有很强的抑制作用，在低浓度和短时间条件下抑制 HL-60细胞的生长，高浓度（2.1～2.7μmol·L -1）及长时间（＞36 h）条件下对细胞具有致死作用，呈时间-剂量依赖性；1.5～2.7μmol·L -1 Leukamenin E处理24 h和48 h均引起了HL-60细胞显著的S期阻滞，同时产生显著的细胞凋亡（P＜0.01），且存在时间-剂量效应；与对照组相比， Leukamenin E处理组产生肾形核，并伴随有多核的产生．
이용태반람배염법검측Leukamenin E대HL-60세포생장적억제작용；재도치현미경급형광현미경하분별검측세포화세포핵형태변화；진일보채용류식세포술화AO/EB형광쌍염법검측Leukamenin E대 HL-60세포주기분포적영향급유도세포조망적작용．결과표명，화합물Leukamenin E대HL-60세포생장유흔강적억제작용，재저농도화단시간조건하억제 HL-60세포적생장，고농도（2.1～2.7μmol·L -1）급장시간（＞36 h）조건하대세포구유치사작용，정시간-제량의뢰성；1.5～2.7μmol·L -1 Leukamenin E처리24 h화48 h균인기료HL-60세포현저적S기조체，동시산생현저적세포조망（P＜0.01），차존재시간-제량효응；여대조조상비， Leukamenin E처리조산생신형핵，병반수유다핵적산생．
T he trypan blue staining is used to detect the proliferation inhibition effect of Leukamenin E on human leukemia HL-60 cells . The changes in morphology of cell and its nucleus are detected under inverted microscope and fluorescence microscope observation . Cell cycle arrest and apoptosis of HL-60 cells induced by Leukamenin E are studied by flow cytometry and AO/EB double staining fluorescence . Results show that Leukamenin E could inhibit the grow th of the HL-60 cells in a low concentration for a short time and also make strong lethal effect at high concentration (2.1~2.7 μmol· L -1 ) for long time (>36h) in a time-and dose-dependent manner ;Leukamenin E induces significantly cell cycle arrest in S phase in HL-60 cells with different concentrations range from 1.5~2.7 μmol·L -1 for 24 h or 48 h;the compound also induces significant apoptosis ( P< 0.01 ) in HL-60 cells in a time- and dose-dependent manner ;compared with control group , renal nucleation and polynucleation come as a very pleasant surprise in treated HL-60 cells .