CAJ | 학술논문

建立了反相高效液相色谱测定杏酒中有机酸类化合物的方法．以金太阳杏为原料，经液体发酵法精心酿制成的低度杏酒作为检测样品，测定了杏酒中9种有机酸类化合物．采用Agilent C18色谱柱（4．6 mm ×250 mm）分离，流动相2％的甲醇-0．01 mol/L （NH4）2 HPO4（pH 2．8）洗脱，流速1 mL/min ，检测器波长为210 nm ，柱温30℃，采用色谱峰保留时间定性，面积外标法定量，并比较了不同杏发酵产品中有机酸的种类和含量，各成分的线性相关系数均大于0．99．方法的回收率大部分都在94％以上，RSD均在3％以下，说明该方法具有快速、分辨率高、重复性好等优点，可用于杏酒中有机酸类化合物的检测．
건립료반상고효액상색보측정행주중유궤산류화합물적방법．이금태양행위원료，경액체발효법정심양제성적저도행주작위검측양품，측정료행주중9충유궤산류화합물．채용Agilent C18색보주（4．6 mm ×250 mm）분리，류동상2％적갑순-0．01 mol/L （NH4）2 HPO4（pH 2．8）세탈，류속1 mL/min ，검측기파장위210 nm ，주온30℃，채용색보봉보류시간정성，면적외표법정량，병비교료불동행발효산품중유궤산적충류화함량，각성분적선성상관계수균대우0．99．방법적회수솔대부분도재94％이상，RSD균재3％이하，설명해방법구유쾌속、분변솔고、중복성호등우점，가용우행주중유궤산류화합물적검측．
RP-HPLC(reversed phase high performance liquid chromatography) method for the determination of organic acids compounds in apricot wine was developed .Low-alcohol apricot wine as samples which was brewed by liquid fermentation with Gold sun apricot ,9 kinds of organic compounds in the apricot wine were determined by retention time of chromatographic peak .The relative determination were summed up as follows :Agilent HC-C18 chromato-graphic column (4 .6 mm × 250 mm ) , mobile phase is 2% methanol-0 .01 mol/L (N H4 )2 HPO4 (pH2 .8) ,the flow rate of 1 mL/min ,detector wavelength of 210 nm ,30 ℃column temperature .Area external standard method as quantitative method ,and compared the type and contents of organic acids in different apricot fermented products .Linear correla-tion coefficient of each component were greater than 0 .99 .The recoveries were above 94% in method ,and RSD were below 3% .The experiments results show that the RP-HPLC method has the advantages of rapid ,high resolution ,good repeatability and can be used for analysis of organic acids compounds in apricot wine .