CAJ | 학술논문

主要采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法（SDS-PAGE）和非变性聚丙烯酰胺凝胶电泳法（Native-PAGE）对新鲜牛羊乳及其酪蛋白清蛋白的区别进行分析检测，对比研究两种电泳方法测定结果的差异，并用两种方法分别检测了掺入不同浓度牛乳的羊乳样品。结果显示SDS-PAGE法对牛羊乳酪白质的分离效果好，该条件下牛羊乳的区别主要是酪蛋白αs2-CN和αs1-CN ，而native-PAGE法则是对清蛋白分离效果较好，该条件下的主要区别是牛乳较羊乳多两条β-乳球蛋白。两种电泳方法对掺入不同浓度的羊乳样品的检测阈值均为5％，但native-PAGE效果更明显。
주요채용십이완기류산납취병희선알응효전영법（SDS-PAGE）화비변성취병희선알응효전영법（Native-PAGE）대신선우양유급기락단백청단백적구별진행분석검측，대비연구량충전영방법측정결과적차이，병용량충방법분별검측료참입불동농도우유적양유양품。결과현시SDS-PAGE법대우양유락백질적분리효과호，해조건하우양유적구별주요시락단백αs2-CN화αs1-CN ，이native-PAGE법칙시대청단백분리효과교호，해조건하적주요구별시우유교양유다량조β-유구단백。량충전영방법대참입불동농도적양유양품적검측역치균위5％，단native-PAGE효과경명현。
This experiment analysis and detection fresh cow and goat milk and casein protein , albumin protein by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and native polyacrylamide gel electrophoresis (native-PAGE) ,comparative the results differ-ences of two electrophoresis methods .And were detected the goat milk samples doped with different concentrations of cow milk by both methods .The results showed that by SDS-PAGE casein protein are separated better ,and the main differences are αs2-CN and αs1-CN , while native-PAGE separate albumin protein better ,and the main difference is that cow milk has two β-lactoglobulin ,but goat milk hasn′t .And the detection threshold of those two kinds of electrophoresis methods of detected the goat milk samples doped with different concentra-tions of cow milk is 5% .But the native-PAGE is more effective .