CAJ | 학술논문

目的：建立并验证后天感染河南樱桃谷鸭乙型肝炎病毒（ DHBV）模型。方法：筛选3日龄DHBV阴性河南樱桃谷鸭，经胫静脉注射DHBV阳性血清建立DHBV模型鸭，连续观察42 d，采用荧光定量PCR方法检测鸭血清和肝脏中DHBV DNA变化情况。 DHBV阴性鸭48只，分为正常组、模型组和3TC组，每组16只。模型组和3TC组于鸭3日龄时建模，模型组给予生理盐水2 mL/（kg· d），3TC组给予3TC 20 mg/（kg· d），连续给药10 d。 PCR检测给药5、10 d后及停药3 d后血清和肝脏中DHBV DNA含量，并进行肝脏病理学观察。结果：感染2 d后，鸭血清和肝脏中均可检测到DHBV DNA。血清DHBV DNA水平第14天达到高峰，拷贝数为7．7×109 mL-1，肝脏DHBV DNA第21天达到高峰，拷贝数达2．1×109 mL-1，42 d内均维持较高的DHBV DNA水平。与模型组比较，3TC组鸭血清和肝脏DHBV DNA拷贝数明显降低（血清：F组间＝17．625，F时间＝68．141，F交互＝134．517，P＜0．001；肝脏：F组间＝69．430，F时间＝35．330，F交互＝75．770，P＜0．001）。3TC组鸭用药10 d后，血清和肝脏HBV DNA抑制率分别为86．5％和84．9％，但停药3 d后有轻度“反弹”现象。3TC组肝组织病变较模型组有明显改善。结论：河南樱桃谷鸭后天感染DHBV较敏感，病毒在体内增殖稳定，是DHBV感染的理想动物模型。
목적：건립병험증후천감염하남앵도곡압을형간염병독（ DHBV）모형。방법：사선3일령DHBV음성하남앵도곡압，경경정맥주사DHBV양성혈청건립DHBV모형압，련속관찰42 d，채용형광정량PCR방법검측압혈청화간장중DHBV DNA변화정황。 DHBV음성압48지，분위정상조、모형조화3TC조，매조16지。모형조화3TC조우압3일령시건모，모형조급여생리염수2 mL/（kg· d），3TC조급여3TC 20 mg/（kg· d），련속급약10 d。 PCR검측급약5、10 d후급정약3 d후혈청화간장중DHBV DNA함량，병진행간장병이학관찰。결과：감염2 d후，압혈청화간장중균가검측도DHBV DNA。혈청DHBV DNA수평제14천체도고봉，고패수위7．7×109 mL-1，간장DHBV DNA제21천체도고봉，고패수체2．1×109 mL-1，42 d내균유지교고적DHBV DNA수평。여모형조비교，3TC조압혈청화간장DHBV DNA고패수명현강저（혈청：F조간＝17．625，F시간＝68．141，F교호＝134．517，P＜0．001；간장：F조간＝69．430，F시간＝35．330，F교호＝75．770，P＜0．001）。3TC조압용약10 d후，혈청화간장HBV DNA억제솔분별위86．5％화84．9％，단정약3 d후유경도“반탄”현상。3TC조간조직병변교모형조유명현개선。결론：하남앵도곡압후천감염DHBV교민감，병독재체내증식은정，시DHBV감염적이상동물모형。
Aim:To establish a standardized duck hepatitis B virus ( DHBV) animal model using Cherry Valley ducks of Henan Province .Methods:3-day-old DHBV negative ducks were selected and injected DHBV positive serum via tibial vein.Fluorescent quantitative polymerase chain reaction ( FQ-PCR) was used to detect the levels of DHBV DNA in serum and liver for 42 days.A total of 48 DHBV negative ducks were allocated into 3 groups:the model group and lamivudine (3TC) group were established DHBV model and given normal saline at 2 mL/(kg· d) and 3TC at 20 mg/(kg· d) for 10 days, respectively.DHBV DNA in the serum and liver were detected after medicating for 5 days, 10 days, and after with-drawal of the drug for 3 days.Histopathological examination of duck liver was also performed .Results:DHBV DNA in ser-um and liver could be detected on the 2nd day of post-infection.The level of DHBV DNA in serum increased to a peak at Day 14 with 7.7 ×109 copies/mL.The level of DHBV DNA in liver increased to a peak at Day 21 with 2.1 ×109 copies/mL.DH-BV DNA maintained a high level in 42 days.3TC [20 mg/(kg· d)] had a strong inhibitory effect on DHBV DNA .The in-hibition rate reached 86.5%and 84.9%in duck serum and liver on Day 10.The histopathological evaluation of the liver also revealed significant improvement .However, a rebound of DHBV DNA level was observed at Day 3 post-treatment.Conclu-sion:Henan Cherry Valley ducks are sensitive to DHBV and the level of virus in the serum and liver is stable , making it an ideal disease model for DHBV infection and can be used for the screening and evaluation of anti -HBV drugs.