郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2013年
6期
783-785
,共3页
赵志鸿%侯迎迎%郑立运%王丽阳%王桂芳%张小俊%张壮丽
趙誌鴻%侯迎迎%鄭立運%王麗暘%王桂芳%張小俊%張壯麗
조지홍%후영영%정립운%왕려양%왕계방%장소준%장장려
艾叶%乙酸乙酯提取物%乙型肝炎病毒%HepG2.2.15细胞
艾葉%乙痠乙酯提取物%乙型肝炎病毒%HepG2.2.15細胞
애협%을산을지제취물%을형간염병독%HepG2.2.15세포
folium artemisiae argyi%ethyl acetate extract%hepatitis B virus%HepG2.2.15 cell
目的:通过体外实验观察艾叶乙酸乙酯提取物对乙型肝炎病毒(简称HBV )的抑制作用。方法:取HepG2.2.15细胞,以1.6、8.0、40.0、200.0和500.0 mg/L的艾叶乙酸乙酯提取物处理72 h后,采用MTT法测定细胞生长抑制率,计算半数毒性浓度。另取HepG2.2.15细胞,分别以0、10、20和40 mg/L艾叶乙酸乙酯提取物及100 mg/L拉米夫定(阳性对照)处理3、6、9 d,采用酶联免疫法检测细胞上清液中乙肝表面抗原、e抗原,用荧光定量PCR测定HBV DNA拷贝数,分别计算抑制率和半数抑制浓度,最后计算治疗指数。结果:艾叶乙酸乙酯提取物对HepG2.2.15细胞的半数毒性浓度为104.80 mg/L,对乙肝表面抗原、e抗原及HBV DNA的半数抑制浓度分别是1.26、8.06和38.97 mg/L,并呈剂量依赖性。治疗指数分别为HBsAg 83.2、e抗原13.0和HBV DNA 2.7。结论:艾叶乙酸乙酯提取物在体外对HBV有明显抑制作用。
目的:通過體外實驗觀察艾葉乙痠乙酯提取物對乙型肝炎病毒(簡稱HBV )的抑製作用。方法:取HepG2.2.15細胞,以1.6、8.0、40.0、200.0和500.0 mg/L的艾葉乙痠乙酯提取物處理72 h後,採用MTT法測定細胞生長抑製率,計算半數毒性濃度。另取HepG2.2.15細胞,分彆以0、10、20和40 mg/L艾葉乙痠乙酯提取物及100 mg/L拉米伕定(暘性對照)處理3、6、9 d,採用酶聯免疫法檢測細胞上清液中乙肝錶麵抗原、e抗原,用熒光定量PCR測定HBV DNA拷貝數,分彆計算抑製率和半數抑製濃度,最後計算治療指數。結果:艾葉乙痠乙酯提取物對HepG2.2.15細胞的半數毒性濃度為104.80 mg/L,對乙肝錶麵抗原、e抗原及HBV DNA的半數抑製濃度分彆是1.26、8.06和38.97 mg/L,併呈劑量依賴性。治療指數分彆為HBsAg 83.2、e抗原13.0和HBV DNA 2.7。結論:艾葉乙痠乙酯提取物在體外對HBV有明顯抑製作用。
목적:통과체외실험관찰애협을산을지제취물대을형간염병독(간칭HBV )적억제작용。방법:취HepG2.2.15세포,이1.6、8.0、40.0、200.0화500.0 mg/L적애협을산을지제취물처리72 h후,채용MTT법측정세포생장억제솔,계산반수독성농도。령취HepG2.2.15세포,분별이0、10、20화40 mg/L애협을산을지제취물급100 mg/L랍미부정(양성대조)처리3、6、9 d,채용매련면역법검측세포상청액중을간표면항원、e항원,용형광정량PCR측정HBV DNA고패수,분별계산억제솔화반수억제농도,최후계산치료지수。결과:애협을산을지제취물대HepG2.2.15세포적반수독성농도위104.80 mg/L,대을간표면항원、e항원급HBV DNA적반수억제농도분별시1.26、8.06화38.97 mg/L,병정제량의뢰성。치료지수분별위HBsAg 83.2、e항원13.0화HBV DNA 2.7。결론:애협을산을지제취물재체외대HBV유명현억제작용。
Methods:HepG2.2.15 cells were allocated into 5 groups and given ethyl acetate extracts at 1.6,8.0,40.0,200.0 and 500 .0 mg/L for 72 h; the inhibition rate of cell growth was determined by MTT and TC50 was calculated .Another HepG2.2.15 cells were allocated into 5 groups and given ethyl acetate extracts at 0,10,20 and 40 mg/L and lamivudine (positive control) at 100 mg/L for 3,6,and 9 days; ELISA was used to determine the contents of HBsAg and HBeAg in cell supernatant , and FQ-PCR was used to determine the HBV DNA copies and the inhibition rate and IC50 were calculat-ed, and then the therapeutic index was calculated accordingly .Results: The TC50 of ethyl acetate extracts was 104 .80 mg/L, and the IC50 for HBsAg, HBeAg and HBV DNA was 1.26,8.06,and 38.97 mg/L,and the therapeutic index for the above 3 indexes was 83.2, 13.0, and 2.7.Conclusion:The extracts from folium artemisiae argyi have obvious inhibitory effects on hepatitis B virus in vitro .