CAJ | 학술논문

目的：探讨脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用。方法：根据所选支架的不同，将实验分为4组，A组为脂肪颗粒，B组为透明质酸钠，C组为Ⅰ型胶原支架，分别按体积比1：1与25×106 mL-1的脂肪来源干细胞混合，D组为单纯脂肪来源干细胞，将4组试剂注射到12只裸鼠皮下构建软组织，每只裸鼠各注射4个点，3个月后获取相应标本，测定湿质量、存活率、微血管密度。结果：4组间湿质量、存活率、微血管密度比较，差异均有统计学意义（F＝47．523、48．425和47．619，P均＜0．05），且A、B、C组湿质量、存活率、微血管密度均高于D组（P均＜0．05）。结论：脂肪来源干细胞复合生物支架可加速软组织血管化，且效果优于单纯脂肪来源干细胞。
목적：탐토지방래원간세포복합불동생물지가재라서피하적촉혈관화작용。방법：근거소선지가적불동，장실험분위4조，A조위지방과립，B조위투명질산납，C조위Ⅰ형효원지가，분별안체적비1：1여25×106 mL-1적지방래원간세포혼합，D조위단순지방래원간세포，장4조시제주사도12지라서피하구건연조직，매지라서각주사4개점，3개월후획취상응표본，측정습질량、존활솔、미혈관밀도。결과：4조간습질량、존활솔、미혈관밀도비교，차이균유통계학의의（F＝47．523、48．425화47．619，P균＜0．05），차A、B、C조습질량、존활솔、미혈관밀도균고우D조（P균＜0．05）。결론：지방래원간세포복합생물지가가가속연조직혈관화，차효과우우단순지방래원간세포。
Aim:To discuss the affection of promoting neovascularization of adipose tissue derived stem cells (ADSCs) compounding with different cellular scaffold in nude mice subcutaneous stratum .Methods:The experiment was divided into four groups depending on the selected scaffold .Group A,B and C were given fat particles, sodium hyaluronate, typeⅠcol-lagen,respectively, mixed with ADSCs at 25 ×106 mL-1 .Group D was given pure ADSCs .Four reagents were injected ran-domly into subcutaneous stratum of 12 nude mice to build soft tissue .Each nude muse was injected 4 points.The appropri-ate specimens were obtained three months later and wet weight , survival rate and microvessel density were detected .Re-sults:The differences were statistically significant regarding the wet weight , survival rate , microvessel density among the four groups(F=47.523,48.425,47.619,P<0.05).The wet weight, survival rate, and microvessel density of group A ,B and C were higher than those of group D (P<0.05).Conclusion: ADSCs compounding with cellular scaffold could pro-mote neovascularization in subcutaneous stratum ,and is better than pure ADSCs .