河南农业科学
河南農業科學
하남농업과학
JOURNAL OF HENAN AGRICULTURAL SCIENCES
2013年
9期
157-160
,共4页
孟衡玲%杨生超%苏一兰%文国松
孟衡玲%楊生超%囌一蘭%文國鬆
맹형령%양생초%소일란%문국송
通关藤%总黄酮%提取工艺
通關籐%總黃酮%提取工藝
통관등%총황동%제취공예
M .tenacissima%flavonoids%extraction process
采用乙醇提取法提取通关藤中的总黄酮,利用正交试验研究提取温度、料液比、提取时间、乙醇体积分数对通关藤中总黄酮提取率的影响。结果表明,影响总黄酮提取率的因素依次为乙醇体积分数>料液比>提取时间>提取温度,最佳工艺为乙醇体积分数70%,料液比1∶20,提取时间3 h ,提取温度70℃,在最佳提取工艺条件下提取率为40.22 m g/g。
採用乙醇提取法提取通關籐中的總黃酮,利用正交試驗研究提取溫度、料液比、提取時間、乙醇體積分數對通關籐中總黃酮提取率的影響。結果錶明,影響總黃酮提取率的因素依次為乙醇體積分數>料液比>提取時間>提取溫度,最佳工藝為乙醇體積分數70%,料液比1∶20,提取時間3 h ,提取溫度70℃,在最佳提取工藝條件下提取率為40.22 m g/g。
채용을순제취법제취통관등중적총황동,이용정교시험연구제취온도、료액비、제취시간、을순체적분수대통관등중총황동제취솔적영향。결과표명,영향총황동제취솔적인소의차위을순체적분수>료액비>제취시간>제취온도,최가공예위을순체적분수70%,료액비1∶20,제취시간3 h ,제취온도70℃,재최가제취공예조건하제취솔위40.22 m g/g。
In this paper ,ethanol extraction was used to obtain total Marsdenia tenacissima fla-vonoids ,with rutin as control sample .The experiment used the orthogonal experimental design method to analyze the optimal extraction process of flavonoids from M .tenacissima .There were four factors affecting extraction efficiency ,i .e .,the material ratio ,extracting time ,extraction tem-perature and mass fraction of ethanol .The results showed that the order of the four factors was :mass fraction of ethanol ,material liquid ratio ,extraction time ,and extraction temperature .The op-timum technology was :the extraction temperature of 70 ℃ ,material liquid ratio of 1∶20 ,mass fraction of ethanol 70% ,and extracting time 3 h .The extraction rate reached 40.22 mg/g in the best extraction conditions .