CAJ | 학술논문

利用C d2＋螯合树脂，对噬菌体随机十二肽库进行C d2＋结合肽筛选，并将亲和力较强的结合肽展示在酵母EB Y 100表面，以期得到耐受和吸附能力具佳的工程菌，为重金属的生物修复提供新的思路。通过筛选共得到6条不同的C d2＋结合肽，其中有2条对C d2＋亲和力较强，将其串联展示于酵母细胞表面。与对照菌相比，酵母工程菌对C d2＋、N i2＋的吸附率显著提高。酵母工程菌对C d2＋的吸附率随诱导时间的增加而增加，诱导24 h时最高，达30.4％，此时酵母工程菌对N i2＋的吸附率达到32．9％。
이용C d2＋오합수지，대서균체수궤십이태고진행C d2＋결합태사선，병장친화력교강적결합태전시재효모EB Y 100표면，이기득도내수화흡부능력구가적공정균，위중금속적생물수복제공신적사로。통과사선공득도6조불동적C d2＋결합태，기중유2조대C d2＋친화력교강，장기천련전시우효모세포표면。여대조균상비，효모공정균대C d2＋、N i2＋적흡부솔현저제고。효모공정균대C d2＋적흡부솔수유도시간적증가이증가，유도24 h시최고，체30.4％，차시효모공정균대N i2＋적흡부솔체도32．9％。
Cd2+ binding peptides were effective enriched from the commercially phage 12 random peptide library ,and six different Cd2+ binding peptides were obtained ,two which had strong affi-nity were selected and connected together in series and displayed on yeast surface by gene recom-bination .The recombinant yeasts showed significant higher adsorption rate for Cd2+ and Ni2+ as compared with the control .The recombinant yeasts adsorption rate for Cd2+ increased with the in-crease of the induction time ,when the induction time was 24 h ,the adsorption rate for Cd2+reached the maximum (30 .4% ) ,and the adsorption rate for Ni2+ reached 32 .9% .