CAJ | 학술논문

脱除蛋白质是真菌多糖纯化过程中较为重要的环节之一，为了优化裂盖马鞍菌多糖脱蛋白质工艺，采用Sevag法和三氯乙酸法对其多糖进行蛋白质脱除，通过比较2种方法处理后的蛋白质脱除率和多糖损失率来确定最佳的脱蛋白质方法。结果表明，三氯乙酸法脱除蛋白质效果最佳，其最佳工艺为三氯乙酸质量浓度50 g/L、三氯乙酸与粗多糖体积比1∶1、振荡时间40 min、静置时间40 min ，在此条件下蛋白质脱除率为92．37％，多糖损失率为16．91％。
탈제단백질시진균다당순화과정중교위중요적배절지일，위료우화렬개마안균다당탈단백질공예，채용Sevag법화삼록을산법대기다당진행단백질탈제，통과비교2충방법처리후적단백질탈제솔화다당손실솔래학정최가적탈단백질방법。결과표명，삼록을산법탈제단백질효과최가，기최가공예위삼록을산질량농도50 g/L、삼록을산여조다당체적비1∶1、진탕시간40 min、정치시간40 min ，재차조건하단백질탈제솔위92．37％，다당손실솔위16．91％。
Deproteinization is one of the most important steps in the purification process of fungi polysaccharide .In order to improve purification efficiency of polysaccharide ,the optimization of deproteinization process of crude polysaccharide from Helvella leucopus Pers .was carried out using Sevag reagent and trichloroacetic acid with protein removal rate and sugar loss rate as in-dexs .The results showed that trichloroacetic acid was more suitable to remove the protein from the crude polysaccharide ,and the optimum trichloroacetic acid concentration was 50 g/L ,the vo-lume ratio of trichloroacetic acid to crude polysaccharide was 1∶1 ,vibrating time and standing time were both 40 min .Under this condition ,the protein removal rate was 92 .37% ,and sugar loss rate w as 16 .91% .