CAJ | 학술논문

为研究太行山羊SPRN基因的结构及其在不同组织中的表达水平，参考绵羊和牛SPRN基因序列设计引物，应用PCR技术获得了太行山羊 S PR N基因的核苷酸序列，同时对该基因进行生物信息学分析，并通过半定量RT PCR进行组织表达谱分析。结果显示，克隆的太行山羊 S PR N基因序列长度为4237 bp ，含有441 bp的完整开放阅读框，编码146个氨基酸，与绵羊和牛的对应基因序列同源性分别达到95％和93％。 S PR N基因在山羊小脑和大脑中表达水平较高，在睾丸、肠系膜淋巴结和肺脏中表达量很低。
위연구태행산양SPRN기인적결구급기재불동조직중적표체수평，삼고면양화우SPRN기인서렬설계인물，응용PCR기술획득료태행산양 S PR N기인적핵감산서렬，동시대해기인진행생물신식학분석，병통과반정량RT PCR진행조직표체보분석。결과현시，극륭적태행산양 S PR N기인서렬장도위4237 bp ，함유441 bp적완정개방열독광，편마146개안기산，여면양화우적대응기인서렬동원성분별체도95％화93％。 S PR N기인재산양소뇌화대뇌중표체수평교고，재고환、장계막림파결화폐장중표체량흔저。
To investigate the gene structure and tissue expression profile of SPRN gene in Taihang goat ,the primers to amplify SPRN gene are designed based on sheep and cattle se-quences .The genomic sequence of caprine SPRN gene was acquired using PCR technology and tissue expre-ssion profile was analyzed using semi-RT-PCR technology .The bioinformatics analy-sis was done based on caprine SPRN gene sequence .The results showed that the length was 4 237 bp , including the complete open reading frame .The full-length of coding sequence was 441 bp ,enco-ding 146 amino acid .The similarity between goat and sheep or cattle was 95% or 93% respective-ly .The expression profile showed that SPRN mRNA was highly expressed in cerebrum and cere-bellum ,low levels in testis ,mesentic lymph node ,and lung and no mRNA was detected in other ti-ssues .The result of phylogenetic analysis was consistent with the taxonomy .The high expression level of SPRN gene in brain tissue is the important basis for the function research .