CAJ | 학술논문

采用遗传转化技术将所要求的外源目的基因导入受体植株，从而创造出人类所需要的新品种是当今科研的一个热点问题，其中，转化方法是植物遗传转化的重要环节。建立一套操作简便、快速、有效的遗传转化体系，是成功获得可遗传性转化植株的关键所在。综述了前人的研究成果，详细总结了以花器官为转化受体的农杆菌介导的植物遗传转化技术的最新研究成果，包括该技术的应用原理、形成过程及优化条件等，并进一步分析了花器官原位转化技术在不同植物中的应用前景。认为，目前可以通过floral spray改良的floral dip及利用drop by drop转化方法获得较高的转化率。但每个方法各有不足。总体来说，floral dip转化方法可进一步改良并推广应用于多种植物种类。
채용유전전화기술장소요구적외원목적기인도입수체식주，종이창조출인류소수요적신품충시당금과연적일개열점문제，기중，전화방법시식물유전전화적중요배절。건립일투조작간편、쾌속、유효적유전전화체계，시성공획득가유전성전화식주적관건소재。종술료전인적연구성과，상세총결료이화기관위전화수체적농간균개도적식물유전전화기술적최신연구성과，포괄해기술적응용원리、형성과정급우화조건등，병진일보분석료화기관원위전화기술재불동식물중적응용전경。인위，목전가이통과floral spray개량적floral dip급이용drop by drop전화방법획득교고적전화솔。단매개방법각유불족。총체래설，floral dip전화방법가진일보개량병추엄응용우다충식물충류。
Successful establishment of a rapid and efficient transformation system is critical to obtain the heritable transformants in the process of genetic engineering .T his paper briefly sketched the previous research results and summarized the recent progresses in detail regarding in-planta transformation of floral organs in situ mediated by A grobacterium in plants ,including the principle of transformation ,formation of methods ,and optimization of experimental perfor-mance as well as analysis of the future application of this in-planta transformation in flowering plants .Floral-spray ,floral-dip and drop-by-drop could get a high transformation rate ,but each has a deficiency .Floral-dip transformation method could be more available to further improve and applied to other species .