中国伤残医学
中國傷殘醫學
중국상잔의학
CHINESE JOURNAL OF TRAUMA AND DISABILITY MEDICINE
2013年
9期
55-57
,共3页
脊髓损伤%Neurogenin2基因%运动功能
脊髓損傷%Neurogenin2基因%運動功能
척수손상%Neurogenin2기인%운동공능
Spinal cord injury%Neurogenin 2 gene%Motor function
目的：研究局部转染Ngn2基因对急性脊髓损伤大鼠运动功能的影响。方法：将36只4月龄SPF级SD大鼠，随机分成3组，分别为A组（假手术组）、B组（实验组）、C组（对照组），每组12只。假手术组只咬除T8～T10棘突及椎板、不损伤脊髓，实验组和对照组采用改良Allen's打击法制造T8急性脊髓损伤模型，术后分别向B、C 2组损伤脊髓节段持续注射Ngn2质粒和空载体质粒各5μg。于术后1、2、3、4周采用BBB运动功能评分系统检测大鼠运动功能，术后1周应用RT-PCR检测损伤节段脊髓组织Ngn2基因表达，术后4周HE染色脊髓病理学检测及SP染色检测Nestin＋细胞数。结果：术后各时间点实验组BBB评分明显优于对照组，有统计学意义（P＜0．05）；实验组大鼠脊髓组织中Ngn2mRNA有表达，而对照组无表达；实验组单个视野Nestin＋细胞为（14．25±2．37）个，对照组单个视野Nestin＋细胞为（2．54±1．14）个，差异有统计学意义（ P＜0．05）。结论：Ngn2基因能够促进急性脊髓损伤大鼠运动功能恢复，可能与诱导脊髓内源性神经干细胞的增殖有关。
目的：研究跼部轉染Ngn2基因對急性脊髓損傷大鼠運動功能的影響。方法：將36隻4月齡SPF級SD大鼠，隨機分成3組，分彆為A組（假手術組）、B組（實驗組）、C組（對照組），每組12隻。假手術組隻咬除T8～T10棘突及椎闆、不損傷脊髓，實驗組和對照組採用改良Allen's打擊法製造T8急性脊髓損傷模型，術後分彆嚮B、C 2組損傷脊髓節段持續註射Ngn2質粒和空載體質粒各5μg。于術後1、2、3、4週採用BBB運動功能評分繫統檢測大鼠運動功能，術後1週應用RT-PCR檢測損傷節段脊髓組織Ngn2基因錶達，術後4週HE染色脊髓病理學檢測及SP染色檢測Nestin＋細胞數。結果：術後各時間點實驗組BBB評分明顯優于對照組，有統計學意義（P＜0．05）；實驗組大鼠脊髓組織中Ngn2mRNA有錶達，而對照組無錶達；實驗組單箇視野Nestin＋細胞為（14．25±2．37）箇，對照組單箇視野Nestin＋細胞為（2．54±1．14）箇，差異有統計學意義（ P＜0．05）。結論：Ngn2基因能夠促進急性脊髓損傷大鼠運動功能恢複，可能與誘導脊髓內源性神經榦細胞的增殖有關。
목적：연구국부전염Ngn2기인대급성척수손상대서운동공능적영향。방법：장36지4월령SPF급SD대서，수궤분성3조，분별위A조（가수술조）、B조（실험조）、C조（대조조），매조12지。가수술조지교제T8～T10극돌급추판、불손상척수，실험조화대조조채용개량Allen's타격법제조T8급성척수손상모형，술후분별향B、C 2조손상척수절단지속주사Ngn2질립화공재체질립각5μg。우술후1、2、3、4주채용BBB운동공능평분계통검측대서운동공능，술후1주응용RT-PCR검측손상절단척수조직Ngn2기인표체，술후4주HE염색척수병이학검측급SP염색검측Nestin＋세포수。결과：술후각시간점실험조BBB평분명현우우대조조，유통계학의의（P＜0．05）；실험조대서척수조직중Ngn2mRNA유표체，이대조조무표체；실험조단개시야Nestin＋세포위（14．25±2．37）개，대조조단개시야Nestin＋세포위（2．54±1．14）개，차이유통계학의의（ P＜0．05）。결론：Ngn2기인능구촉진급성척수손상대서운동공능회복，가능여유도척수내원성신경간세포적증식유관。
Objective:To investigate the effect of local Ngn 2 plasmid transfer on motor function in rats with acute spinal cord injury motor function.Methods:36 four-month-old SPF SD rats were randomly divided into three groups ,namely Group A (sham group), Group B (experimental group), Group C (control group),n =12.Sham group was only bite T8-T10 spinous process and lamina with-out damage to the spinal cord , the experimental group and the control group made the T 8 acute spinal cord injury model by using modified Allen's method.Postoperatively, 5μg Ngn2 plasmid for Group B and 5μg vector plasmid for Group C were continuously injected into the spinal cord segments .1,2,3,4 weeks after surgery to detect motor function in rats using the BBB motor function scoring system , 1 week after surgery to test the expression of Ngn 2 gene of spinal cord injury tissue by RT -PCR and 4 weeks after surgery detect spinal pathology by HE staining or the number of Nestin +cells by SP staining .Results:Postoperative BBB score the experimental group at each time point was significantly better than the control group .It was statistically significant (P <0.05).There was the expression of Ngn2 gene in spinal cord injury tissue of the experimental group , no expression in the control group .The experimental group had Nestin +cells (14. 25 ±2.37) ones per a single vision, while the control group Nestin +cells (2.54 ±1.14) ones, the difference was statistically sig-nificant (P <0.05).Conclusion:Ngn2 gene transferred by local injection has significant effect on the motor function recovery in rats with acute spinal cord injury , which may induce proliferation of the endogenous neural stem cells in the spinal cord .
