CAJ | 학술논문

建立用自动固相萃取/高效液相色谱法测定西瓜和香蕉中的甲霜灵残留的方法，样品经乙腈提取，过滤，自动固相萃取，采用高效液相色谱检测，色谱柱：SunFireTM C18；流动相：乙腈-水-甲醇(体积比25∶40∶35)；流速：1.0 mL/min；检测波长：220 nm；进样量：10μL。该方法在西瓜和香蕉中添加0.02、0.2、1.0 mg/kg 3个不同水平加标，回收率分别为94.3%～101.0%和81.5%～99.6%，相对标准偏差在1.30%～5.29%和2.66%～6.67%。该方法的最低检出限为0.006 mg/kg，满足农药残留检测的要求。
건립용자동고상췌취/고효액상색보법측정서과화향초중적갑상령잔류적방법，양품경을정제취，과려，자동고상췌취，채용고효액상색보검측，색보주：SunFireTM C18；류동상：을정-수-갑순(체적비25∶40∶35)；류속：1.0 mL/min；검측파장：220 nm；진양량：10μL。해방법재서과화향초중첨가0.02、0.2、1.0 mg/kg 3개불동수평가표，회수솔분별위94.3%～101.0%화81.5%～99.6%，상대표준편차재1.30%～5.29%화2.66%～6.67%。해방법적최저검출한위0.006 mg/kg，만족농약잔류검측적요구。
A method for determination of metalaxyl (MEL) in water melons and bananas have been developed by automatic solid-phase extraction (SPE) coupled with high performance liquid chromatography (HPLC). Samples were firstly extracted by acetonitrile and then homogenized, filtrated, automatic solid-phase extraction (SPE), finally purified by C18 column. The experiment was carried on SunFireTM C18 column with the mobile phase of acetonitrile-water-methanol (25∶40∶35, by vol) at the flow rate of 1.0 mL/min. The detection wavelength and injection volume were set at 220nm and 10uL respectively. The recoveries of three different concentration (0.02, 0.20, 1.00 mg/kg) for water melons and bananas were between 94.3%~101.0%and 81.5%~99.6%, respectively. RSD (n=5) were 1.30%~5.29%and 2.66%~6.67%, respectively. The lower detection limit for metalaxyl (MEL) was 0.006mg/kg. This method met with the requirements of the pesticide residue detection.