CAJ | 학술논문

利用全自动生化分析仪，对人工淡水驯养3龄、8～10龄和14～16龄等3个年龄组的子一代中华鲟以及性腺发育至Ⅲ期的野生中华鲟共计四个类别中华鲟的蛋白质、血脂、血糖、血清酶类和无机离子等18项血液生化指标进行测定，以探究人工环境条件下子一代中华鲟的生物学特性。结果显示，随着人工养殖子一代中华鲟年龄的增加，血蛋白（血清总蛋白、白蛋白、球蛋白）和钠离子的含量增加，血脂（总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇）的含量呈“先降后升”趋势，血糖的含量呈“先升后降”趋势，血清酶类（谷草转氨酶、肌酸激酶、谷丙转氨酶、乳酸脱氢酶和碱性磷酸酶）的活性以3龄子一代中华鲟个体最高。野生Ⅲ期中华鲟有9项指标（氯离子、肌酸激酶、碱性磷酸酶、血清总蛋白、白蛋白、球蛋白、总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇）显著低于与其性腺发育状况相同的14～16龄人工养殖中华鲟（ P＜0．05），二者在其他9项血液生化指标间无显著性差异。研究表明，中华鲟的血液生化指标与其生理特点、生活环境、身体状况和性腺发育程度具有明显的相关性。
이용전자동생화분석의，대인공담수순양3령、8～10령화14～16령등3개년령조적자일대중화심이급성선발육지Ⅲ기적야생중화심공계사개유별중화심적단백질、혈지、혈당、혈청매류화무궤리자등18항혈액생화지표진행측정，이탐구인공배경조건하자일대중화심적생물학특성。결과현시，수착인공양식자일대중화심년령적증가，혈단백（혈청총단백、백단백、구단백）화납리자적함량증가，혈지（총담고순、감유삼지、고밀도지단백담고순、저밀도지단백담고순）적함량정“선강후승”추세，혈당적함량정“선승후강”추세，혈청매류（곡초전안매、기산격매、곡병전안매、유산탈경매화감성린산매）적활성이3령자일대중화심개체최고。야생Ⅲ기중화심유9항지표（록리자、기산격매、감성린산매、혈청총단백、백단백、구단백、총담고순、고밀도지단백담고순화저밀도지단백담고순）현저저우여기성선발육상황상동적14～16령인공양식중화심（ P＜0．05），이자재기타9항혈액생화지표간무현저성차이。연구표명，중화심적혈액생화지표여기생리특점、생활배경、신체상황화성선발육정도구유명현적상관성。
Eighteen serum indices including protein ,fat ,glucose ,and inorganic ion levels and enzyme activity were monitored in 3 years old ,8~10 years old ,14~16 years old farmed F1 Chinese sturgeon (Acipenser sinensis) and the Chinese sturgeon with Ⅰ ,Ⅱ and Ⅲ stage gonads domesticated in artificial freshwater environment by automatic biochemical analyzer (type:7170A of Hitachi) to study the biological characteristics of Chinese sturgeon .The results showed that the levels of serum protein including total protein (TP) ,albumin(ALB) ,globulin (GLOB) and Na+ wereshown to be ascendant trend .The levels of serum fat including total cholesterol (CHOL) ,triglyceride (TRIG) ,high density lipoprotein cholesterol (HDLC) and low density lipoprotein cholesterol (LDLC ) were found to be decreased firstly ,and then increased as the time was elapsed while the concentration of serum glucose (GLU) was raised first and then decreased . The maximal activities of serum enzymes including glutamine-oxaloacetate (AST ) , creatine kinase (CK ) , glutamate-pyruvate transaminase (GPT ) , lactate dehydrogenase (LDH ) and alkaline phosphatase (ALP) were observed in the 3 year old individuals of F1 Chinese sturgeon .There were significantly lower 9 blood indices(Cl- ,CK ,ALP ,TP ,ALB ,GLOB ,CHOL ,HDLC and LDLC)in the wild Chinese sturgeon than in 14 ~ 16 years old group of farmed F1 Chinese sturgeon with same developmental gonads (P<0 .05) ,even though the wild Chinese sturgeon showed stage Ⅲ gonads .No significant differences in the other blood indices were found in the wild and farmed Chinese sturgeon (P>0 .05 ) . T he findings indicate that the significant differences in blood biochemistry characteristics are involved in the inherent background variability of these sturgeon individuals ,including exposure history in different habitats ,different developmental gonad and physiological characteristics .