热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2013年
10期
72-76
,共5页
椰子胚乳%乙酰辅酶A羧化酶%高效液相色谱
椰子胚乳%乙酰輔酶A羧化酶%高效液相色譜
야자배유%을선보매A최화매%고효액상색보
coconut endosperm%ACCase activity%HPLC
建立利用HPLC测定椰子胚乳乙酰辅酶A羧化酶(ACCase)活性的方法。色谱柱为德国MN nucleosil C18(4.60 mm i.d×250 mm，5.0μm)，流动相为10 mmol/L磷酸二氢钾(pH 6.7)︰甲醇＝90︰10(V︰V )。结果表明： Malonyl-CoA浓度在0.02～0.12μg/mL，色谱方法有良好的线性关系，线性相关系数为0.999， Malonyl-CoA最低检测浓度为0.02μg/mL，最终测定椰子胚乳ACCase活性为1.20 ng Malonyl-CoA/min/μg酶蛋白。
建立利用HPLC測定椰子胚乳乙酰輔酶A羧化酶(ACCase)活性的方法。色譜柱為德國MN nucleosil C18(4.60 mm i.d×250 mm，5.0μm)，流動相為10 mmol/L燐痠二氫鉀(pH 6.7)︰甲醇＝90︰10(V︰V )。結果錶明： Malonyl-CoA濃度在0.02～0.12μg/mL，色譜方法有良好的線性關繫，線性相關繫數為0.999， Malonyl-CoA最低檢測濃度為0.02μg/mL，最終測定椰子胚乳ACCase活性為1.20 ng Malonyl-CoA/min/μg酶蛋白。
건립이용HPLC측정야자배유을선보매A최화매(ACCase)활성적방법。색보주위덕국MN nucleosil C18(4.60 mm i.d×250 mm，5.0μm)，류동상위10 mmol/L린산이경갑(pH 6.7)︰갑순＝90︰10(V︰V )。결과표명： Malonyl-CoA농도재0.02～0.12μg/mL，색보방법유량호적선성관계，선성상관계수위0.999， Malonyl-CoA최저검측농도위0.02μg/mL，최종측정야자배유ACCase활성위1.20 ng Malonyl-CoA/min/μg매단백。
A HPLC method for determining ACCase activity in coconut endosperm was established. Chromatographic column was Germany MN nucleosil C18 (4.60 mm i.d × 250 mm, 5.0 μm). The mobile phase was 10.0 mM KH2PO4 (pH 6.7) and methyl alcohol at the proportion of 90∶10 (V∶V ). Results showed the calibration curve of malonyl-CoA had a good linear correlation in the range of 0.02μg/mL~0.12 μg/mL; the linear relative coefficient was 0.999. The limit of detection was 0.02 μg/mL. Finally ACCase activity in coconut endosperm was 1.20 ng malonyl-CoA/min/g extract proteins.
