贵州医药
貴州醫藥
귀주의약
GUIZHOU MEDICAL JOURNAL
2013年
10期
867-873
,共7页
李在楠%严瑞%聂梦琪%朱春玲
李在楠%嚴瑞%聶夢琪%硃春玲
리재남%엄서%섭몽기%주춘령
Toll样受体4%转化生长因子-β1%肾间质纤维化%黑骨藤追风活络胶囊
Toll樣受體4%轉化生長因子-β1%腎間質纖維化%黑骨籐追風活絡膠囊
Toll양수체4%전화생장인자-β1%신간질섬유화%흑골등추풍활락효낭
Hei guteng zhui feng huo luo capsule%Toll-like receptor4%Transforming grow th factor-β1%Tubulointerstitial fibrosis
目的：探讨Toll样受体4（toll-like receptor 4，TLR4）在肾间质纤维化大鼠模型中的表达及意义；研究黑骨藤追风活络胶囊对肾间质纤维化的作用及可能的机制。方法采用单侧输尿管梗阻（UUO）致肾间质纤维化大鼠模型。将36只雄性SD大鼠随机分为假手术组、模型组、黑骨藤追风活络胶囊（HGT ）治疗组（243 mg/kg/d），手术后第7天、第14天分别处死各组中6只大鼠。用HE染色方法观察各组大鼠肾小管间质损伤程度，免疫组化方法观察各组大鼠肾组织中 TLR4、转化生长因子-β1（transforming growth factor-β1，TGF-β1）的表达情况。结果模型组 TLR4、TGF-β1的表达及肾小管间质损伤指数明显高于同期假手术组（P＜0．01）；黑骨藤追风活络胶囊治疗组 TLR4、TGF-β1的表达及肾小管间质损伤指数明显低于模型组（P＜0．01）。结论 TLR4及其介导的信号通路可能在肾间质纤维化中起促进作用；黑骨藤追风活络胶囊对肾间质纤维化有一定的防治作用。
目的：探討Toll樣受體4（toll-like receptor 4，TLR4）在腎間質纖維化大鼠模型中的錶達及意義；研究黑骨籐追風活絡膠囊對腎間質纖維化的作用及可能的機製。方法採用單側輸尿管梗阻（UUO）緻腎間質纖維化大鼠模型。將36隻雄性SD大鼠隨機分為假手術組、模型組、黑骨籐追風活絡膠囊（HGT ）治療組（243 mg/kg/d），手術後第7天、第14天分彆處死各組中6隻大鼠。用HE染色方法觀察各組大鼠腎小管間質損傷程度，免疫組化方法觀察各組大鼠腎組織中 TLR4、轉化生長因子-β1（transforming growth factor-β1，TGF-β1）的錶達情況。結果模型組 TLR4、TGF-β1的錶達及腎小管間質損傷指數明顯高于同期假手術組（P＜0．01）；黑骨籐追風活絡膠囊治療組 TLR4、TGF-β1的錶達及腎小管間質損傷指數明顯低于模型組（P＜0．01）。結論 TLR4及其介導的信號通路可能在腎間質纖維化中起促進作用；黑骨籐追風活絡膠囊對腎間質纖維化有一定的防治作用。
목적：탐토Toll양수체4（toll-like receptor 4，TLR4）재신간질섬유화대서모형중적표체급의의；연구흑골등추풍활락효낭대신간질섬유화적작용급가능적궤제。방법채용단측수뇨관경조（UUO）치신간질섬유화대서모형。장36지웅성SD대서수궤분위가수술조、모형조、흑골등추풍활락효낭（HGT ）치료조（243 mg/kg/d），수술후제7천、제14천분별처사각조중6지대서。용HE염색방법관찰각조대서신소관간질손상정도，면역조화방법관찰각조대서신조직중 TLR4、전화생장인자-β1（transforming growth factor-β1，TGF-β1）적표체정황。결과모형조 TLR4、TGF-β1적표체급신소관간질손상지수명현고우동기가수술조（P＜0．01）；흑골등추풍활락효낭치료조 TLR4、TGF-β1적표체급신소관간질손상지수명현저우모형조（P＜0．01）。결론 TLR4급기개도적신호통로가능재신간질섬유화중기촉진작용；흑골등추풍활락효낭대신간질섬유화유일정적방치작용。
Objective To investigate the expression and significance of Toll-like receptor 4 (TLR4) in rat model of Tubulointerstitial fibrosis .To investigate the effect and mechanism of drug which were hei guteng zhui feng huo luo capsule (HGT ) on the process of the fibrosis .Methods 36 normal male Sprague-Dawley rats were randomly divided into sham-operated controls ,unilateral ureter-al obstruction (UUO) ,HGT treatment (243 mg/d) ,6 rats of each group were sacrificed randomly on the 7 days ,14 days after surgery .The tubulointerstitial damage indexe of histological studies were im-plemented by HE .Immuno-histochemistry was performed on obstructed renal tissue to determine the expression of TLR4 and transforming growth factor-β1 (TGF-β1) .Results The expressions of TLR4 and TGF-β1 and the tubulointerstitial damage indexs in UUO group at different time points were sig-nificantly higher than that in sham-operated group (P<0 .01) .The expressions of TLR4 and TGF-β1 in HGT group were significantly lower than that in UUO group (P<0 .01) ,also the tubulointerstitial damage indexs .Conclusion TLR4 with its signal transduction progress may play a stimulative role in renal interstitial fibrosis .HGT can cure and prevent from renal interstitial fibrosis .
