CAJ | 학술논문

采用毛细管气相色谱法测定眼科手术粘弹剂中乳酸的含量。乳酸与甲醇酯化反应后，选用氯仿提取其中酯类物质，进行GC分析。采用DB-Wax毛细管柱(30 mm×0.32 mm，0.5μm)，FID检测器，进样口温度为250℃，检测器温度为250℃。采用程序升温，起始温度为70℃，以5℃/min升温至100℃，再以25℃/min升至220℃，保持2 min。在选择的色谱条件下，酯化后的乳酸得到良好的分离，乳酸线性范围为0.0894～1.1652 mg/mL (r=0.9996)，检出限为0.15μg/mL。方法的加标回收率为97.8％～98.8％，测定结果的相对标准偏差为1.97%(n=6)。该方法样品前处理简单，干扰少，重现性好，分离效率高，可作为眼科手术粘弹剂中功能成分乳酸质量控制的方法。
채용모세관기상색보법측정안과수술점탄제중유산적함량。유산여갑순지화반응후，선용록방제취기중지류물질，진행GC분석。채용DB-Wax모세관주(30 mm×0.32 mm，0.5μm)，FID검측기，진양구온도위250℃，검측기온도위250℃。채용정서승온，기시온도위70℃，이5℃/min승온지100℃，재이25℃/min승지220℃，보지2 min。재선택적색보조건하，지화후적유산득도량호적분리，유산선성범위위0.0894～1.1652 mg/mL (r=0.9996)，검출한위0.15μg/mL。방법적가표회수솔위97.8％～98.8％，측정결과적상대표준편차위1.97%(n=6)。해방법양품전처리간단，간우소，중현성호，분리효솔고，가작위안과수술점탄제중공능성분유산질량공제적방법。
The method for the determination of lactic acid in ophthalmologic surgical viscoelastic agent by capillaty gas chromatography was established. After the esterication of lactic acid with methanol, chloroform was used to extract the esterication product for GC analysis. ADB-Wax column (30 mm×0.32 mm, 0.5 μm) and FID were used. The injector and detector temperature was 250℃. Column temperature was controlled by the program:initial temperature of 70℃was raised to 100℃at 5℃/min, then raised to 220℃(2 min) at 25℃/min. Under the optimised conditions, esterification of lactic acid was well separated. Standard addition test results showed that the recoveries were 97.8%-98.8%, the relative standard deviation was 1.97%(n=6). The detection limit was 0.15 μg/mL. This method is simple,fast,sensitive and accurate,and it can be used for mass control of lactic acid content in ophthalmologic surgical viscoelastic agents.