世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
5期
1201-1204
,共4页
羊胡髭草%大披针薹草%抗炎%利尿%急性毒性
羊鬍髭草%大披針薹草%抗炎%利尿%急性毒性
양호자초%대피침대초%항염%이뇨%급성독성
Herb of Lanceolate Sedge%Carex lanceolata Boott%anti-inflammatory%dieresis%acute toxicity
目的：研究羊胡髭草的抗炎利尿作用，并进行相关急性毒性实验。方法：小鼠随机分为5组：生理盐水组、阿司匹林组（0.05 g·kg-1）、羊胡髭草水提液高、中、低剂量组[10、5、2.5 g（生药）·kg-1]，灌胃给药，每天1次，连续灌胃3天后，采用二甲苯致小鼠耳廓肿胀法造模，计算耳廓肿胀度及肿胀抑制率；另取大鼠随机分为5组：生理盐水组、阿司匹林组（0.05 g·kg-1）、羊胡髭草水提液高、中、低剂量组[10、5、2.5 g（生药）·kg-1]，灌胃给药，每天1次，连续灌胃5天后，采用角叉菜胶致大鼠足趾肿胀法造模，计算足趾肿胀率；另取大鼠随机分为5组：生理盐水组、呋塞米片组（0.01 g·kg-1）、羊胡髭草水提液高、中、低剂量组[10、5、2.5 g（生药）·kg-1]，灌胃给药，每天1次，连续5天，采用水负荷方法造模，计算5 h内尿量；采用最大耐受量（MTD）测定其急性毒性。结果：羊胡髭草水提液高、中、低3个剂量组对小鼠耳廓肿胀度、肿胀抑制率、大鼠足趾肿胀率均有明显的抑制作用，对大鼠5 h内尿量有明显增加作用；小鼠羊胡髭草水提液灌胃给药MTD为640 g（生药）·kg-1。结论：羊胡髭草对实验动物模型有明显的抗炎利尿作用，且急性毒性很小。
目的：研究羊鬍髭草的抗炎利尿作用，併進行相關急性毒性實驗。方法：小鼠隨機分為5組：生理鹽水組、阿司匹林組（0.05 g·kg-1）、羊鬍髭草水提液高、中、低劑量組[10、5、2.5 g（生藥）·kg-1]，灌胃給藥，每天1次，連續灌胃3天後，採用二甲苯緻小鼠耳廓腫脹法造模，計算耳廓腫脹度及腫脹抑製率；另取大鼠隨機分為5組：生理鹽水組、阿司匹林組（0.05 g·kg-1）、羊鬍髭草水提液高、中、低劑量組[10、5、2.5 g（生藥）·kg-1]，灌胃給藥，每天1次，連續灌胃5天後，採用角扠菜膠緻大鼠足趾腫脹法造模，計算足趾腫脹率；另取大鼠隨機分為5組：生理鹽水組、呋塞米片組（0.01 g·kg-1）、羊鬍髭草水提液高、中、低劑量組[10、5、2.5 g（生藥）·kg-1]，灌胃給藥，每天1次，連續5天，採用水負荷方法造模，計算5 h內尿量；採用最大耐受量（MTD）測定其急性毒性。結果：羊鬍髭草水提液高、中、低3箇劑量組對小鼠耳廓腫脹度、腫脹抑製率、大鼠足趾腫脹率均有明顯的抑製作用，對大鼠5 h內尿量有明顯增加作用；小鼠羊鬍髭草水提液灌胃給藥MTD為640 g（生藥）·kg-1。結論：羊鬍髭草對實驗動物模型有明顯的抗炎利尿作用，且急性毒性很小。
목적：연구양호자초적항염이뇨작용，병진행상관급성독성실험。방법：소서수궤분위5조：생리염수조、아사필림조（0.05 g·kg-1）、양호자초수제액고、중、저제량조[10、5、2.5 g（생약）·kg-1]，관위급약，매천1차，련속관위3천후，채용이갑분치소서이곽종창법조모，계산이곽종창도급종창억제솔；령취대서수궤분위5조：생리염수조、아사필림조（0.05 g·kg-1）、양호자초수제액고、중、저제량조[10、5、2.5 g（생약）·kg-1]，관위급약，매천1차，련속관위5천후，채용각차채효치대서족지종창법조모，계산족지종창솔；령취대서수궤분위5조：생리염수조、부새미편조（0.01 g·kg-1）、양호자초수제액고、중、저제량조[10、5、2.5 g（생약）·kg-1]，관위급약，매천1차，련속5천，채용수부하방법조모，계산5 h내뇨량；채용최대내수량（MTD）측정기급성독성。결과：양호자초수제액고、중、저3개제량조대소서이곽종창도、종창억제솔、대서족지종창솔균유명현적억제작용，대대서5 h내뇨량유명현증가작용；소서양호자초수제액관위급약MTD위640 g（생약）·kg-1。결론：양호자초대실험동물모형유명현적항염이뇨작용，차급성독성흔소。
This study was aimed to investigate the anti-inflammatory, diuretic effect and acute toxicity of Herb of Lanceolate Sedge. The mice were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was given to mice once a day for 3 days. Auricular swelling induced by dimethyl benzene was used in the model establishment to calculate the swollen ear degree and the swollen inhibiting rate. And rats were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of wa-ter extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medi-cation was given to rats once a day for 5 days. Carrageenan induced paw edema was used in the model establishment to calculate the paw swollen rate. And rats were randomly divided into 5 groups, which were the physiological saline group, Furosemide tablets group (0.01 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was giv-en to rats once a day for 5 days. Water-loaded was used in the model establishment to calculate the 5-hour urine amount. The maximum tolerance dose (MTD) was used in the determination of the acute toxicity. The results showed that the three groups of HD, MD and LD of water extract of Herb of Lanceolate Sedge had obvious inhibiting effects on the swollen ear degree and the swollen inhibiting rate among mice, and the paw swollen rate of rats. It had signif-icant increasing effect on the 5-hour urine amount. The MTD of intragastric administration of water extract of Herb of Lanceolate Sedge was 640 g crude drug per kg for mice. It was concluded that Herb of Lanceolate Sedge dis-played obvious effects on anti-inflammatory and dieresis with low acute toxicity.
