化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2014年
3期
50-52
,共3页
苯并a芘%高效液相色谱法%二极管阵列检测器%生活饮用水
苯併a芘%高效液相色譜法%二極管陣列檢測器%生活飲用水
분병a비%고효액상색보법%이겁관진렬검측기%생활음용수
benzo (a) pyrene%high performance liquid chromatograph%PDA(photo-diode array) detector%drinking water
建立高效液相色谱二极管阵列检测器检测生活饮用水苯并[a]芘的测定方法。采用C18反相色谱柱(150 mm×4.6 mm，5μm)，在流动相为甲醇-水(体积比为90∶10)、流量1.0 mL/min、检测波长295 nm、柱温35℃、进样体积20μL的条件下测定生活饮用水中苯并[a]芘。该方法检出限为6 ng/L，线性范围0～100 ng/mL，加标回收率为88.1%～93.4%，测定结果的相对标准偏差为1.06%(n=9)。该法样品预处理简单，分离度高，分析时间短，适用于生活饮用水中苯并[a]芘的准确定性定量测定。
建立高效液相色譜二極管陣列檢測器檢測生活飲用水苯併[a]芘的測定方法。採用C18反相色譜柱(150 mm×4.6 mm，5μm)，在流動相為甲醇-水(體積比為90∶10)、流量1.0 mL/min、檢測波長295 nm、柱溫35℃、進樣體積20μL的條件下測定生活飲用水中苯併[a]芘。該方法檢齣限為6 ng/L，線性範圍0～100 ng/mL，加標迴收率為88.1%～93.4%，測定結果的相對標準偏差為1.06%(n=9)。該法樣品預處理簡單，分離度高，分析時間短，適用于生活飲用水中苯併[a]芘的準確定性定量測定。
건립고효액상색보이겁관진렬검측기검측생활음용수분병[a]비적측정방법。채용C18반상색보주(150 mm×4.6 mm，5μm)，재류동상위갑순-수(체적비위90∶10)、류량1.0 mL/min、검측파장295 nm、주온35℃、진양체적20μL적조건하측정생활음용수중분병[a]비。해방법검출한위6 ng/L，선성범위0～100 ng/mL，가표회수솔위88.1%～93.4%，측정결과적상대표준편차위1.06%(n=9)。해법양품예처리간단，분리도고，분석시간단，괄용우생활음용수중분병[a]비적준학정성정량측정。
A method was developed for determining benzo [a] pyrene in drinking water by HPLC-PDA detector. With column of C18(150 mm×4.6 mm,5μm),the mobile phase of methanol-water(volume ratio was 90∶10) and the flow rate of 1.0 mL/min,the determine wavelength of 295 nm,the column temperature of 35℃,and the injection volume of 20μL, the concentration of benzo [a] pyrene in drinking water was detected. The limit of detection was 6 ng/mL. The linear plots were obtained between 0-100 ng/mL. Overall recoveries were between 88.1%and 93.4%,and the relative standard deviation of determination results was 1.06%(n=9). This method has advantages of simple process of sample treatment, good isolation and short analysis time,and is suitable for the quantitative and qualitative determination of Benzo [a] pyrene in drinking water.
