CAJ | 학술논문

目的：考察新疆紫草脂质体凝胶剂对放射性皮肤损伤创面及愈合相关因素的影响。方法用不同体积的脂质加入到适量的凝胶基质中制备脂质体凝胶剂，考察其体外释药特性；以直线加速器β射线照射Wistar大鼠后臀部，建立皮肤放射性损伤模型，观察模型用药后的愈合时间、创面组织病理改变、皮肤羟脯氨酸（hy p ）含量、血清MDA、SOD、IL-6、TGF-β变化情况。结果脂质体凝胶剂组（AELG）体外释药曲线符合 Higuchi方程，透皮速率比普通凝胶低。40 Gy剂量单次照射后20 d左右出现脱毛、溃烂现象。经照射后皮肤组织中羟脯氨酸含量，血清中MDA、IL-6、TGF-β水平明显下降，SOD含量显著升高（P ＜0．05）。创面用药后，各给药组溃疡4～5 w基本愈合，愈合时间较阴性组明显缩短（P ＜0．05）。皮肤组织中羟脯氨酸含量所升高，血清中MDA、IL-6、TGF-β含量升高，SOD含量下降，脂质体凝胶剂效果优于普通凝胶剂。结论新疆紫草脂质体凝胶剂对放射性皮肤损伤的愈合有一定的促进作用，其机理可能与促进胶原蛋白合成有关。
목적：고찰신강자초지질체응효제대방사성피부손상창면급유합상관인소적영향。방법용불동체적적지질가입도괄량적응효기질중제비지질체응효제，고찰기체외석약특성；이직선가속기β사선조사Wistar대서후둔부，건립피부방사성손상모형，관찰모형용약후적유합시간、창면조직병리개변、피부간포안산（hy p ）함량、혈청MDA、SOD、IL-6、TGF-β변화정황。결과지질체응효제조（AELG）체외석약곡선부합 Higuchi방정，투피속솔비보통응효저。40 Gy제량단차조사후20 d좌우출현탈모、궤란현상。경조사후피부조직중간포안산함량，혈청중MDA、IL-6、TGF-β수평명현하강，SOD함량현저승고（P ＜0．05）。창면용약후，각급약조궤양4～5 w기본유합，유합시간교음성조명현축단（P ＜0．05）。피부조직중간포안산함량소승고，혈청중MDA、IL-6、TGF-β함량승고，SOD함량하강，지질체응효제효과우우보통응효제。결론신강자초지질체응효제대방사성피부손상적유합유일정적촉진작용，기궤리가능여촉진효원단백합성유관。
Objective The study is to investigate the effect of Arnebia euchroma (Royle) Johnst .extracts li-posomes gel(AELG )on radiation-induced skin injury and related factors in rats .Methods The AELG were prepared by mixing the liposomes of extracts from Arnebia euchroma (Royle) Johnst .with gel base in different volume ,and the transdermal profile were tested ;the model of radiation-induced skin injury were made on the hip of rats under the β-ray from linear accelerator .The healing time of ulcer ,pathological changes of tissue ,contents of hydroxyproline( hyp) in skin tissue ,serium level of MDA ,SOD ,IL-6 and TGF-βwere tested ,respectively .Results The releasing profile in viro .of gel corresponded to the Higuchi equation and the releasing rate were lowering with the increasing of liposomes amounts .The ulcer of skin appeared after 20 days′exposure under radiation of 40gry once ,and the contents of hyp ,serum level of MDA ,IL-6 and TGF-βwere decreased ,but SOD increased significantly .Being administrated the prepara-tion of gel and AELG on the ulcer of skin ,the ulcer healed within 4 -5 weeks ,the healing time of both groups which were administrated with gel were shorter than negative group .The contents of hyp ,level of MDA ,IL-6 and TGF-βwere increased ,SOD were decreased ,the AELG groups were better than the gel groups in terms of extents of indexes above .Conclusion AELG could promote the healing of radiation-in-duced skin ulcer .The mechanism may be related to the enhancement of collagen protein synthetization .