CAJ | 학술논문

目的：探讨胃肠道间质瘤（gastrointestinal stormal tumors ，GISTs）中磷酸化细胞外信号调节激酶（phosphorylated extracellular signal regulated kinase ，p-ERK1/2）、细胞周期蛋白D1（CyclinD1）和增殖细胞核抗原（proliferating cell nuclear antigen ，PCNA）蛋白的表达及其与临床病理因素的关系。方法采用免疫组织化学方法检测50例GISTs患者及肿瘤旁正常组织（平滑肌）中p-ERK1/2、CyclinD1和PCNA的表达水平，并进行比较分析。结果 GISTs组织中p-ERK1/2、CyclinD1和PCNA蛋白阳性表达率（82．0％、62．0％、66．0％）明显高于肿瘤旁正常组织（24．0％、30．0％、38．0％），差异有统计学意义（P ＜0．05）。随着肿瘤直径增大，p-ERK1/2、CyclinD1和PCNA蛋白阳性表达率逐渐增高，差异有统计学意义（P ＜0．05）。不同性别的GISTs患者CyclinD1蛋白的表达差异有统计学意义（P ＜0．05）。不同年龄、民族及发生部位的GISTs患者p-ERK1/2、CyclinD1和PCNA蛋白的表达差异无统计学意义（P ＞0．05）。相关性分析显示，p-ERK1/2（r＝0．291，P＜0．05）和PCNA （r＝0．378， P＜0．05）蛋白表达与肿瘤分级呈正相关。结论 ERK1/2激活、CyclinD1和PCNA高表达对肿瘤细胞的增殖、浸润和转移有一定的促进作用。
목적：탐토위장도간질류（gastrointestinal stormal tumors ，GISTs）중린산화세포외신호조절격매（phosphorylated extracellular signal regulated kinase ，p-ERK1/2）、세포주기단백D1（CyclinD1）화증식세포핵항원（proliferating cell nuclear antigen ，PCNA）단백적표체급기여림상병리인소적관계。방법채용면역조직화학방법검측50례GISTs환자급종류방정상조직（평활기）중p-ERK1/2、CyclinD1화PCNA적표체수평，병진행비교분석。결과 GISTs조직중p-ERK1/2、CyclinD1화PCNA단백양성표체솔（82．0％、62．0％、66．0％）명현고우종류방정상조직（24．0％、30．0％、38．0％），차이유통계학의의（P ＜0．05）。수착종류직경증대，p-ERK1/2、CyclinD1화PCNA단백양성표체솔축점증고，차이유통계학의의（P ＜0．05）。불동성별적GISTs환자CyclinD1단백적표체차이유통계학의의（P ＜0．05）。불동년령、민족급발생부위적GISTs환자p-ERK1/2、CyclinD1화PCNA단백적표체차이무통계학의의（P ＞0．05）。상관성분석현시，p-ERK1/2（r＝0．291，P＜0．05）화PCNA （r＝0．378， P＜0．05）단백표체여종류분급정정상관。결론 ERK1/2격활、CyclinD1화PCNA고표체대종류세포적증식、침윤화전이유일정적촉진작용。
Objective To explore the expressions of p-ERK1/2 ,cyclinD1 and PCNA proteins in gastrointes-tinal stromal tumors (GISTs) and find out their relationships with clinicopathological factors .Methods p-ERK1/2 ,CyclinD1 and PCNA protein expressions in GISTs and normal tissues of 50 patients were detec-ted by using immunohistochemistry method ,and the results were analized .Results The expressions of p-ERK1/2 ,CyclinD1 and PCNA protein in GISTs tissues (82 .0% ,62 .0% ,66 .0% ) were significantly high-er than those in adjacent normal tissues (24 .0% ,30 .0% ,38 .0% ) ,with the difference statistically signifi-cant (P<0 .05) .There are statistically significant differences in the CyclinD1 protein expression of differ-ent gender of patients with GISTs (P <0 .05) .There are no statistically significant differences in the ex-pression of different ages ,nationalities and locations of GISTs in patients with p-ERK1/2 ,CyclinD1 and PCNA protein (P >0 .05) .As the tumor increases ,p-ERK1/2 ,CyclinD1 and PCNA gradually increased , and the difference was statistically significant (P <0 .05) .Meanwhile ,correlation analysis showed that p-ERK1/2 (r = 0 .291 ,P <0 .05) and PCNA (r = 0 .378 ,P <0 .05) proteins expression were positively correlated with the degree of cancer risk .Conclusion ERK1/2 activation ,CyclinD1 and PCNA high ex-pression have a certain role in the proliferation ,invasion and metastasis of tumor cell .