CAJ | 학술논문

目的：测定新疆不同地区和品种的葡萄树伤流液中氨基酸含量，为葡萄树伤流液药用、食用价值提供理论依据。方法采自新疆和田市、吐鲁番鄯善县、喀什市和阿图什市的9种葡萄树伤流液，采用异硫氰酸苯酯（PITC）为柱前衍生化试剂衍生化后进行高效液相色谱（HPLC）分析，建立葡萄树伤流液中17种氨基酸含量测定方法，并对其特征氨基酸进行评价。结果9个品种的葡萄树伤流液中氨基酸有17种，总含量为6．20～201．22 m g/L ，其中7种必需氨基酸。主成分分析得出组氨酸、酪氨酸、缬氨酸、异亮氨酸、苯丙氨酸、天冬氨酸和丙氨酸是葡萄树伤流液的特征氨基酸。结论不同品种葡萄树伤流液的氨基酸含量差异较大，但是各类氨基酸含量分布与组成类似。主成分分析法是葡萄树伤流液氨基酸含量分析的有效方法。
목적：측정신강불동지구화품충적포도수상류액중안기산함량，위포도수상류액약용、식용개치제공이론의거。방법채자신강화전시、토로번선선현、객십시화아도십시적9충포도수상류액，채용이류청산분지（PITC）위주전연생화시제연생화후진행고효액상색보（HPLC）분석，건립포도수상류액중17충안기산함량측정방법，병대기특정안기산진행평개。결과9개품충적포도수상류액중안기산유17충，총함량위6．20～201．22 m g/L ，기중7충필수안기산。주성분분석득출조안산、락안산、힐안산、이량안산、분병안산、천동안산화병안산시포도수상류액적특정안기산。결론불동품충포도수상류액적안기산함량차이교대，단시각류안기산함량분포여조성유사。주성분분석법시포도수상류액안기산함량분석적유효방법。
Objective To determine the amino acid content of different kinds bleeding sap of grape tree from different areas of Xinjiang ,and provide a theoretical basis on medicinal and food value of bleeding sap of grape trees in Xinjiang .Methods Derivated by phenylisothiocyanate (PITC) ,the determination on 17 kinds of amino acids in 9 grapevine bleeding sap was established ,which were collected from Hotan ,Tur-pan and Atushi .The characteristic amino acids were evaluated .Results There were 16 kinds of amino acids in bleeding sap of Xinjiang grape tree and their contents were 6 .20~201 .22 mg/L ,and 7 of them were essential for human body .The analysis results of principal components showed that histidine ,tyro-sine ,valine ,isoleucine ,phenylalanine ,asparticacid may be the characteristic amino acids .Conclusions There was significant difference between contents of various amino acids ,but their distribution and compo-sition were similar .Principal component analysis is an effective way to analyze the amino acid contents in bleeding sap of grape trees .