CAJ | 학술논문

目的：研究分析喀什木纳格葡萄树伤流液中多糖的分离纯化及其单糖组成成分。方法采用醇沉法提取多糖，Sevage法脱蛋白，经DEAE-52纤维素及SephedexG-150凝胶柱分离纯化，采用气相色谱分析法测定单糖组成。结果80％乙醇沉淀、Sevage法除蛋白得粗多糖；葡萄树伤流液粗多糖经过DEAE-52分离纯化分别得到GBSK-1、GBSK-2、GBSK-3、GBSK-4、GBSK-5、GBSK-6、GBSK-7和GBSK-8共8个多糖组分。其中4个多糖组分经过SephadexG-150纯化后，其洗脱峰均为单峰；通过GC分析得出GBSK-3的单糖组成较简单，主要由木糖组成；多糖GBSK-2、GBSK-5的单糖组成相似，主要由鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖组成；多糖GBSK-1的单糖组成主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖组成。结论该方法较好地分离纯化葡萄树伤流液多糖。GC分析适用于多糖中的单糖组成分析，为葡萄树伤流液的开发和利用提供了科学依据。
목적：연구분석객십목납격포도수상류액중다당적분리순화급기단당조성성분。방법채용순침법제취다당，Sevage법탈단백，경DEAE-52섬유소급SephedexG-150응효주분리순화，채용기상색보분석법측정단당조성。결과80％을순침정、Sevage법제단백득조다당；포도수상류액조다당경과DEAE-52분리순화분별득도GBSK-1、GBSK-2、GBSK-3、GBSK-4、GBSK-5、GBSK-6、GBSK-7화GBSK-8공8개다당조분。기중4개다당조분경과SephadexG-150순화후，기세탈봉균위단봉；통과GC분석득출GBSK-3적단당조성교간단，주요유목당조성；다당GBSK-2、GBSK-5적단당조성상사，주요유서리당、아랍백당、감로당、포도당、반유당조성；다당GBSK-1적단당조성주요유서리당、아랍백당、목당、감로당、포도당、반유당조성。결론해방법교호지분리순화포도수상류액다당。GC분석괄용우다당중적단당조성분석，위포도수상류액적개발화이용제공료과학의거。
Objective To separate and purify of Kaxigar munaka grapevine bleeding sap polysaccharides and analysis of monosaccharide composition .Methods Prepare crude polysaccharide using alcohol precipitation and by improved Sevage method .Purify the obtained crude polysaccharide by DEAE-52 and Sephadex G-150 column chromatography .Analysis the monosaccharide composition by gas chromatography .Results To get crude polysaccharide by 80% alcohol precipitation and to remove proteins by Sevage method .The crude polysaccharide was separated by DEAE-52 ,and obtained 8 polysaccharides ,respectively GBSK-1 , GBSK-2 ,GBSK-3 ,GBSK-4 ,GBSK-5 ,GBSK-6 ,GBSK-7 and GBSK-8 .After being purified ,4 polysaccha-rides eluting peaks were unimodal .From the result obtained by GC analysis ,monosaccharide composition of GBSK-3 was relatively simple and mainly composed of xylose .The polysaccharides GBSK-2 ,GBSK-5 had similar monosaccharide composition and they were mainly composed of rhamnose ,arabinose ,man-nose ,glucose and galactose .While the polysaccharides GBSK-1 was mainly composed of rhamnose ,arabi-nose ,xylose ,mannose ,glucose and galactose .Conclusion Results indicate that this method is suitable for the separation and purification of grapevine bleeding sap polysaccharide .It is suitable to analysis the monosaccharide composition of grapevine bleeding sap polysaccharides by gas chromatography .A scientific basis is provided for the development and utilization of grapevine bleeding sap .