CAJ | 학술논문

目的：探讨原发性胃腺癌（gastric adenocarcinoma, GAC）组织中CD133、CD44和E-cadherin蛋白表达的关系及临床病理意义。方法应用免疫组织化学ElivisionTM plus法检测145例胃腺癌组织和30例正常胃黏膜组织中CD133、CD44和E-cadherin蛋白的表达。结果在正常胃黏膜组织中，CD133、CD44和E-cadherin蛋白的阳性表达率分别为10.0%、3.3%和100%；在GAC组中，CD133、CD44和E-cadherin蛋白的阳性表达率分别为46.9%、47.6%和42.8%，两组之间，差异均有显著性（P<0.05）。CD133、CD44和E-cadherin蛋白的表达水平均与肿瘤的大小、分化程度、淋巴结转移与否、浸润深度、临床分期及术后生存期有关（全部P<0.05）；CD133的表达与CD44的表达呈正相关（P<0.05），CD133和CD44的表达均与E-cadherin的表达呈负相关（P<0.005）。Kaplan-Meier生存分析表明，CD133和CD44蛋白表达阳性的患者其生存率明显低于其阴性表达患者（P<0.001）；E-cadherin蛋白表达阳性的患者其生存率明显高于其阴性表达患者。Cox回归分析：CD133、CD44和E-cadherin的表达是影响GAC患者术后生存率的独立预后因素。结论 CD133、CD44和E-cadherin的表达水平在GAC组织中与肿瘤组织的大小、分化程度、临床分期、浸润深度、淋巴结转移与否及预后等均有关；在GAC组织中联合检测CD133、CD44和E-cadherin对判断预后有价值。
목적：탐토원발성위선암（gastric adenocarcinoma, GAC）조직중CD133、CD44화E-cadherin단백표체적관계급림상병리의의。방법응용면역조직화학ElivisionTM plus법검측145례위선암조직화30례정상위점막조직중CD133、CD44화E-cadherin단백적표체。결과재정상위점막조직중，CD133、CD44화E-cadherin단백적양성표체솔분별위10.0%、3.3%화100%；재GAC조중，CD133、CD44화E-cadherin단백적양성표체솔분별위46.9%、47.6%화42.8%，량조지간，차이균유현저성（P<0.05）。CD133、CD44화E-cadherin단백적표체수평균여종류적대소、분화정도、림파결전이여부、침윤심도、림상분기급술후생존기유관（전부P<0.05）；CD133적표체여CD44적표체정정상관（P<0.05），CD133화CD44적표체균여E-cadherin적표체정부상관（P<0.005）。Kaplan-Meier생존분석표명，CD133화CD44단백표체양성적환자기생존솔명현저우기음성표체환자（P<0.001）；E-cadherin단백표체양성적환자기생존솔명현고우기음성표체환자。Cox회귀분석：CD133、CD44화E-cadherin적표체시영향GAC환자술후생존솔적독립예후인소。결론 CD133、CD44화E-cadherin적표체수평재GAC조직중여종류조직적대소、분화정도、림상분기、침윤심도、림파결전이여부급예후등균유관；재GAC조직중연합검측CD133、CD44화E-cadherin대판단예후유개치。
Objective To explore the expression of CD133 and CD44 in the primary gastric adenocarcinoma (GAC) and their relationship with the expression of E-cadherin. Methods The expressions of CD133, CD44, and E-cadherin was examined by immunohistochemistry in 145 specimens of GAC tissues and 30 specimens of normal gastric tissues. Results The positivity rates of CD133, CD44, and E-cadherin in normal gastric tissues were 10.0%, 0%, and 100%, respectively, showing significant differences from the rates in GAC tissues (46.9%, 47.6%, and 42.8%, respectively) (P<0.05). The expressions of CD133, CD44, and E-cadherin were significantly related with the tumor volume, differentiation, lymph node metastasis, invasive depth, pathologic-tumor-node-metastasis (pTNM) stages, and postoperative-survival time (all P<0.05); a positive correlation was found between the expression of CD133 and CD44, and they were both negatively correlated with E-cadherin expression (P<0.005). Kaplan-Meier analysis showed a significant difference in the survival rate between CD133-positive and CD133-negative patients (P<0.001) and between CD44-positive and CD44-negative patients;the patients with positive expression of E-cadherin had a significantly longer survival than those negative for E-cadherin. Cox regression analysis indicated that CD133, CD44, and E-cadherin expressions were all independent prognostic factors of GAC (all P<0.05). Conclusion The expressions of CD133, CD44, and E-cadherin are related to the tumor volume, differentiation, pTNM stages, invasive depth, lymph node metastasis, and prognosis of GAC, and their combined detection can be of important value in predicting the prognosis of GAC.