CAJ | 학술논문

目的：探讨应用电穿孔技术对DNA疫苗pVAX-tG250FcGB免疫效果的影响。方法构建肾癌DNA疫苗pVAX-tG250FcGB，通过体内电穿孔技术或普通肌肉注射途径，将DNA疫苗导入到BALB/c小鼠体内，探讨电穿孔免疫途径诱导抗原特异性免疫应答的效果。结果电穿孔技术或普通肌肉注射均能在小鼠体内诱导出抗原特异性的体液免疫应答和细胞免疫应答，电穿孔技术的免疫效果明显优于普通肌肉注射途径。结论电穿孔技术介导的DNA疫苗pVAX-tG250FcGB免疫具有较强的诱导免疫应答的能力，是研究DNA疫苗pVAX-tG250FcGB免疫效果的一种有效途径。
목적：탐토응용전천공기술대DNA역묘pVAX-tG250FcGB면역효과적영향。방법구건신암DNA역묘pVAX-tG250FcGB，통과체내전천공기술혹보통기육주사도경，장DNA역묘도입도BALB/c소서체내，탐토전천공면역도경유도항원특이성면역응답적효과。결과전천공기술혹보통기육주사균능재소서체내유도출항원특이성적체액면역응답화세포면역응답，전천공기술적면역효과명현우우보통기육주사도경。결론전천공기술개도적DNA역묘pVAX-tG250FcGB면역구유교강적유도면역응답적능력，시연구DNA역묘pVAX-tG250FcGB면역효과적일충유효도경。
Objective To investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAX-tG250FcGB. Methods The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared. Results The vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection. Conclusion Electroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.