CAJ | 학술논문

目的：探讨黄芪注射液对 SD大鼠心肌缺血再灌注损伤（MIRI）中Smad3、Smad7表达的影响和机制。方法采用结扎左冠状动脉的方法制备心肌缺血再灌注损伤动物模型。32只健康成年 SD大鼠，体质量（230±20）g，平均分为假手术组、缺血再灌注组、辛伐他汀组和黄芪预处理组。通过免疫组织化学和 RT-PCR，分别测定 Smad3蛋白、Smad7蛋白及Smad3 mRNA、Smad7 mRNA的表达。计算各组中心肌细胞的凋亡率。结果与假手术组相比，缺血再灌注组心肌细胞的凋亡率明显增加（P＝0．000），并且 Smad3蛋白和Smad3 mRNA表达增加（P＝0．000），Smad7蛋白和Smad7 mRNA表达减少（P＝0．000）；与缺血再灌注组相比，黄芪注射液组（P＝0．000）和辛伐他汀组（P＝0．000）可明显降低心肌细胞的凋亡率；Smad3蛋白和Smad3 mR-NA表达减少（P＝0．000）；而Smad7蛋白和Smad7 mRNA表达增加（P＝0．000）。结论黄芪注射液可以上调SD大鼠缺血再灌注损伤时心肌细胞 Smad7蛋白和Smad7 mRNA表达，下调 Smad3蛋白和Smad3 mRNA表达。
목적：탐토황기주사액대 SD대서심기결혈재관주손상（MIRI）중Smad3、Smad7표체적영향화궤제。방법채용결찰좌관상동맥적방법제비심기결혈재관주손상동물모형。32지건강성년 SD대서，체질량（230±20）g，평균분위가수술조、결혈재관주조、신벌타정조화황기예처리조。통과면역조직화학화 RT-PCR，분별측정 Smad3단백、Smad7단백급Smad3 mRNA、Smad7 mRNA적표체。계산각조중심기세포적조망솔。결과여가수술조상비，결혈재관주조심기세포적조망솔명현증가（P＝0．000），병차 Smad3단백화Smad3 mRNA표체증가（P＝0．000），Smad7단백화Smad7 mRNA표체감소（P＝0．000）；여결혈재관주조상비，황기주사액조（P＝0．000）화신벌타정조（P＝0．000）가명현강저심기세포적조망솔；Smad3단백화Smad3 mR-NA표체감소（P＝0．000）；이Smad7단백화Smad7 mRNA표체증가（P＝0．000）。결론황기주사액가이상조SD대서결혈재관주손상시심기세포 Smad7단백화Smad7 mRNA표체，하조 Smad3단백화Smad3 mRNA표체。
Objective To explore the effect and mechanism of astragalus injection on Smad3 and Smad7 expression caused by myocardial ischemia reperfusion inj ury(MIRI)in SD Rats.Methods The MIRI model was established by ligating the left anterior descending coronary artery.Thirty two SD rats were randomly and equally divided into four groups named by sham,model,simvas-tatin and astragalus pretreatment after one week′s adaptive feeding.The protein and mRNA expressions of Smad3 and Smad7 were respectively detected by immunohistochemical staining and RT-PCR in cardiomyocyte,in order to analysis the relationship between Smad3′s and Smad7′s protein and mRNA expressions and calculate each group′s myocardial cell apoptosis rate.Results Compared with the Sham group,ischemia reperfusion myocardial cells apoptosis rate increased significantly(P=0.000),the protein and mR-NA expression of Smad3 increased(P=0.000)but the Smad7 decreased(P=0.000).Compared with the ischemia reperfusion,as-tragalus injection group and simvastatin group can significantly reduce the apoptosis of myocardial cells(P=0.000),The protein and mRNA expression of Smad3 decreased(P=0.000)but the Smad7 increased(P=0.000).Conclusion Astragalus injection can affect the apoptosis of myocardial cells in ischemia reperfusion inj ury of the SD rat myocardial cells,which is realized by up-regula-ting Smad7 protein and Smad7mRNA expression,down-regulating Smad3 protein and Smad3mRNA expression.