中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
14期
6487-6490
,共4页
二异丙酚%催眠药和镇静药%认知障碍%糖基化终产物,高级
二異丙酚%催眠藥和鎮靜藥%認知障礙%糖基化終產物,高級
이이병분%최면약화진정약%인지장애%당기화종산물,고급
Propofol%Hypnotics and sedatives%Cognition disorders%Glycosylation end products,advanced
目的：本研究拟观察丙泊酚长期镇静对大鼠认知功能及海马高级糖基化终末产物受体（RAGE）蛋白表达的影响及认知功能改变与海马RAGE蛋白表达有无关系，为临床提供依据。方法（1）选取成年雄性Wistar大鼠12只，随机分为对照组、丙泊酚组。（2）丙泊酚组每天连续输注8 h，连续给药5 d，对照组给予生理盐水。应用Morris水迷宫对两组大鼠开始连续5 d的行为学观察，记录逃逸潜伏期和空间探索时间。（3）大鼠末次水迷宫测试1 h后，取大鼠海马，分别用ELISA法和免疫组织化学法测定RAGE蛋白的表达。结果（1）定位航行试验：①与对照组比较，丙泊酚组在第1～4天逃逸潜伏期延长（P<0.05）；②对照组4个时点的两两比较显示：与第1天比较第2、4天逃逸潜伏期缩短（P<0.05）。丙泊酚组在4个时点的两两比较显示：与第1天比较第4天逃逸潜伏期缩短（P<0.05）。（2）空间探索试验：与对照组比较，丙泊酚组空间探索时间缩短，穿越平台次数减少（P<0.05）。（3）海马 RAGE 表达：ELISA 法测定 RAGE蛋白结果，与对照组比较，丙泊酚组海马 RAGE 蛋白表达上调（P<0.05）；（4）免疫组化法测定 RAGE 蛋白结果：与对照组比较，异丙酚组海马CA1区RAGE免疫组化染色阳性细胞数增加。结论丙泊酚长期镇静可损害成年大鼠的空间学习记忆能力，导致成年大鼠认知功能降低，可能与其上调海马RAGE表达有关。
目的：本研究擬觀察丙泊酚長期鎮靜對大鼠認知功能及海馬高級糖基化終末產物受體（RAGE）蛋白錶達的影響及認知功能改變與海馬RAGE蛋白錶達有無關繫，為臨床提供依據。方法（1）選取成年雄性Wistar大鼠12隻，隨機分為對照組、丙泊酚組。（2）丙泊酚組每天連續輸註8 h，連續給藥5 d，對照組給予生理鹽水。應用Morris水迷宮對兩組大鼠開始連續5 d的行為學觀察，記錄逃逸潛伏期和空間探索時間。（3）大鼠末次水迷宮測試1 h後，取大鼠海馬，分彆用ELISA法和免疫組織化學法測定RAGE蛋白的錶達。結果（1）定位航行試驗：①與對照組比較，丙泊酚組在第1～4天逃逸潛伏期延長（P<0.05）；②對照組4箇時點的兩兩比較顯示：與第1天比較第2、4天逃逸潛伏期縮短（P<0.05）。丙泊酚組在4箇時點的兩兩比較顯示：與第1天比較第4天逃逸潛伏期縮短（P<0.05）。（2）空間探索試驗：與對照組比較，丙泊酚組空間探索時間縮短，穿越平檯次數減少（P<0.05）。（3）海馬 RAGE 錶達：ELISA 法測定 RAGE蛋白結果，與對照組比較，丙泊酚組海馬 RAGE 蛋白錶達上調（P<0.05）；（4）免疫組化法測定 RAGE 蛋白結果：與對照組比較，異丙酚組海馬CA1區RAGE免疫組化染色暘性細胞數增加。結論丙泊酚長期鎮靜可損害成年大鼠的空間學習記憶能力，導緻成年大鼠認知功能降低，可能與其上調海馬RAGE錶達有關。
목적：본연구의관찰병박분장기진정대대서인지공능급해마고급당기화종말산물수체（RAGE）단백표체적영향급인지공능개변여해마RAGE단백표체유무관계，위림상제공의거。방법（1）선취성년웅성Wistar대서12지，수궤분위대조조、병박분조。（2）병박분조매천련속수주8 h，련속급약5 d，대조조급여생리염수。응용Morris수미궁대량조대서개시련속5 d적행위학관찰，기록도일잠복기화공간탐색시간。（3）대서말차수미궁측시1 h후，취대서해마，분별용ELISA법화면역조직화학법측정RAGE단백적표체。결과（1）정위항행시험：①여대조조비교，병박분조재제1～4천도일잠복기연장（P<0.05）；②대조조4개시점적량량비교현시：여제1천비교제2、4천도일잠복기축단（P<0.05）。병박분조재4개시점적량량비교현시：여제1천비교제4천도일잠복기축단（P<0.05）。（2）공간탐색시험：여대조조비교，병박분조공간탐색시간축단，천월평태차수감소（P<0.05）。（3）해마 RAGE 표체：ELISA 법측정 RAGE단백결과，여대조조비교，병박분조해마 RAGE 단백표체상조（P<0.05）；（4）면역조화법측정 RAGE 단백결과：여대조조비교，이병분조해마CA1구RAGE면역조화염색양성세포수증가。결론병박분장기진정가손해성년대서적공간학습기억능력，도치성년대서인지공능강저，가능여기상조해마RAGE표체유관。
Objective To observe the effect of long-term sedation of propofol on cognitive function in rats and expression of receptors of advanced glycation end products(RAGE)in the hippocampus, and to explore the correlation between cognitive function and hippocampal expression of RAGE, in order to provide a basis for clinical. Methods (1) 12 adult male Wistar rats were selected and randomly divided into control group and propofol group. (2) Continuous infusion of 8 hours per day, given 5 days in a row, the control group were given saline solution. Two groups of rats were used Morris water maze began continuous behavioral observation of 5 days to record escape latency time and space exploration. (3)After the last water maze test in rats 1 h, the rat hippocampus were taked, respectively, by means of ELISA and immunohistochemical determination of expression of RAGE. Results (1) Positioning navigation:①each time point 4 comparison between two groups:compared with the control group, the remaining three escape d1-4 incubation period was extended; ②Compared with d1, escape incubation period were shortened in d2-4 in control group(P<0.05);In propofol group, compared with d1, escape incubation period were shortened in d4 (P<0.05);(2) Test results of space exploration:compared with the control group, space exploration time were shortened and crossed the platform were less in propofol group. (3) Determination of RAGE protein ELISA results:compared with the control group, expression of RAGE protein was increased in hippocampus in propofol group; (4)Immunohistochemical results:compared with the control group, dyeing positive cell number increased in CA1 area of hippocampus in propofol group. Conclusions Effects of propofol lytic long calm can damage of rat spatial learning and memory abilities. The effects of propofol can cause a reduction in cognitive function in adult rats, may be associated with their increase in hippocampal expression of RAGE.
