中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
14期
6457-6461
,共5页
巨细胞%黑色素瘤%肿瘤转移%小鼠
巨細胞%黑色素瘤%腫瘤轉移%小鼠
거세포%흑색소류%종류전이%소서
Giant cells%Melanoma%Neoplasm metastasis%Mice
目的：研究多核巨细胞在黑色素瘤转移中的作用及其相应分子机制。方法用聚乙二醇细胞融合法体外获取黑色素瘤多核巨细胞，通过流式分选黑色素瘤多核巨细胞。小鼠皮下及尾静脉注射黑色素瘤多核巨细胞并用游标卡尺测量皮下肿瘤体积，电子天平称重荷瘤肺组织以定量肿瘤转移能力。Affymatrix基因芯片分析差异表达的基因，Ingenuity Pathways Analysis（IPA）软件进行基因功能分析。结果细胞融合并流式分选成功获得黑色素瘤多核巨细胞。黑色素瘤多核巨细胞皮下生长速度减慢但肺转移能力明显增强。黑色素瘤多核巨细胞与转移相关的β-Tubulin组基因表达明显升高。黑色素瘤多核巨细胞的染色体呈现稳定性。结论黑色素瘤多核巨细胞促进黑色素瘤细胞转移能力，黑色素瘤多核巨细胞与转移相关的β-Tubulin组基因表达升高。
目的：研究多覈巨細胞在黑色素瘤轉移中的作用及其相應分子機製。方法用聚乙二醇細胞融閤法體外穫取黑色素瘤多覈巨細胞，通過流式分選黑色素瘤多覈巨細胞。小鼠皮下及尾靜脈註射黑色素瘤多覈巨細胞併用遊標卡呎測量皮下腫瘤體積，電子天平稱重荷瘤肺組織以定量腫瘤轉移能力。Affymatrix基因芯片分析差異錶達的基因，Ingenuity Pathways Analysis（IPA）軟件進行基因功能分析。結果細胞融閤併流式分選成功穫得黑色素瘤多覈巨細胞。黑色素瘤多覈巨細胞皮下生長速度減慢但肺轉移能力明顯增彊。黑色素瘤多覈巨細胞與轉移相關的β-Tubulin組基因錶達明顯升高。黑色素瘤多覈巨細胞的染色體呈現穩定性。結論黑色素瘤多覈巨細胞促進黑色素瘤細胞轉移能力，黑色素瘤多覈巨細胞與轉移相關的β-Tubulin組基因錶達升高。
목적：연구다핵거세포재흑색소류전이중적작용급기상응분자궤제。방법용취을이순세포융합법체외획취흑색소류다핵거세포，통과류식분선흑색소류다핵거세포。소서피하급미정맥주사흑색소류다핵거세포병용유표잡척측량피하종류체적，전자천평칭중하류폐조직이정량종류전이능력。Affymatrix기인심편분석차이표체적기인，Ingenuity Pathways Analysis（IPA）연건진행기인공능분석。결과세포융합병류식분선성공획득흑색소류다핵거세포。흑색소류다핵거세포피하생장속도감만단폐전이능력명현증강。흑색소류다핵거세포여전이상관적β-Tubulin조기인표체명현승고。흑색소류다핵거세포적염색체정현은정성。결론흑색소류다핵거세포촉진흑색소류세포전이능력，흑색소류다핵거세포여전이상관적β-Tubulin조기인표체승고。
Objective To study the role of melanoma multinucleated giant cells in tumor metastasis and the corresponding molecular mechanism. Methods Melanoma multinucleated giant cells were obtained through the polyethylene glycol(PEG)fusion method following fluorescence activated cell sorting (FACS). To detect the tumor growth rate in vivo, subcutaneous tumor volumes were measured using a vernier caliper. To analyze the tumor metastasis potential, cells were injected intravenously, and the collected lung metastasis samples were weighed by an electronic balance. Differentially expressed genes between the melanoma multinucleated giant cells and parental cells were detected by the Affymatrix gene chip following the gene function analysis by Ingenuity Pathways Analysis(IPA). Results Melanoma multinucleated giant cells were successfully obtained through PEG cell fusion method following FACS. The metastasis potential of melanoma multinucleated giant cells was significantly enhanced, in contrast to their subcutaneously decreased growth rate. A group of β-Tubulin gene related to metastasis was significantly up regulated in melanoma multinucleated giant cells. Melanoma multinucleated giant cells exhibited genomic stability. Conclusion Melanoma multinucleated giant cells acquired enhanced metastasis potential,andβ-Tubulin genes related to melanoma metastasis were up regulated.
