CAJ | 학술논문

目的：研究纳米二氧化钛（TiO2）对健康SD大鼠及氧化应激SD大鼠肺组织的毒性效应。方法将48只健康雄性SD大鼠随机分为4组，分别为健康对照组（Control）、TiO2纳米材料处理组（NM组）、四氧嘧啶处理组（OS组）和四氧嘧啶及TiO2纳米材料共同处理组（OS-NM组），每组12只。采用气管滴注方式注入低、中、高剂量TiO2对NM组和OS-NM进行染毒。测定大鼠血气分析指标（氧分压、氧饱和度和血氧含量）；肺组织病理切片分析各组肺组织的损伤情况。结果与健康大鼠相比，氧化应激组大鼠的氧分压、氧饱和度和血氧含量显著降低，肺组织低张性缺氧加剧。肺组织病理切片分析表明，相同剂量的纳米材料处理后，氧化应激状态加剧了纳米材料的生物毒性，肺组织中出现大量炎细胞浸润、肺泡壁毛细血管高度扩张充血及肺泡结构消失。结论氧化应激加剧了纳米材料对肺组织的损伤，机体的疾病状态与纳米材料之间存在协同效应。
목적：연구납미이양화태（TiO2）대건강SD대서급양화응격SD대서폐조직적독성효응。방법장48지건강웅성SD대서수궤분위4조，분별위건강대조조（Control）、TiO2납미재료처리조（NM조）、사양밀정처리조（OS조）화사양밀정급TiO2납미재료공동처리조（OS-NM조），매조12지。채용기관적주방식주입저、중、고제량TiO2대NM조화OS-NM진행염독。측정대서혈기분석지표（양분압、양포화도화혈양함량）；폐조직병리절편분석각조폐조직적손상정황。결과여건강대서상비，양화응격조대서적양분압、양포화도화혈양함량현저강저，폐조직저장성결양가극。폐조직병리절편분석표명，상동제량적납미재료처리후，양화응격상태가극료납미재료적생물독성，폐조직중출현대량염세포침윤、폐포벽모세혈관고도확장충혈급폐포결구소실。결론양화응격가극료납미재료대폐조직적손상，궤체적질병상태여납미재료지간존재협동효응。
Objective To study the influence of nano-titanium dioxide(TiO2) on the lung in rats. Methods 48 healthy male SD rats were randomly divided into four groups, namely the healthy control group(Control), TiO2 nanomaterials treatment group(NM group), alloxan treated group(OS group)and alloxan and TiO2 Nano material common treatment group(OS-NM group), each TiO2 was given to rats by tracheal instillation. The levels of oxygen partial pressure, oxygen saturation and oxygen content in blood were determined. Lung tissue damage was analyzed by lung tissue biopsy analysis. Results Compared with the control, rats oxidative stress oxygen partial pressure, oxygen saturation and blood oxygen content was significantly reduced, hypotonic hypoxia was intensified in lung tissue. Pulmonary histopathological analysis showed that the same dose of nano-materials processing, oxidative stress exacerbates toxicity of nanomaterials, lung tissue showed a large number of inflammatory cell infiltration, alveolar wall and alveolar capillary dilatation and congestion height structure disappeared. Conclusion oxidative stress exacerbates the of damage nanomaterials on lung tissue, there are synergies between the body of the disease state and nanomaterials.