CAJ | 학술논문

目的：对两种质谱方法ESI-LC-MS/MS和APCI-LC-MS/MS测定人血清25羟基维生素D进行比较。方法建立了两种人血清25羟基维生素D浓度的测定方法，通过准确度、精密度、线性范围等参数对方法进行了验证，并分别测定了50例实际血清样本。结果两种方法的定量限均满足测定要求；线性范围均为2.1～158.0 ng/ml（25羟基维生素D3）、2.1～152.0 ng/ml（25羟基维生素D2）；实际样本加标回收率均在85%～115%；精密度，ESI方法优于APCI法；实际血清样本检测结果的t检验分析表明两种方法无显著性差异；仪器清洗维护频率比较显示，APCI-LC-MS/MS方法复杂很多。结论两种方法在实际检测过程中可相互替代，但为减少仪器日常清洗及维护频率，获得更稳定的检测结果，建议选择ESI-LC-MS/MS法。
목적：대량충질보방법ESI-LC-MS/MS화APCI-LC-MS/MS측정인혈청25간기유생소D진행비교。방법건립료량충인혈청25간기유생소D농도적측정방법，통과준학도、정밀도、선성범위등삼수대방법진행료험증，병분별측정료50례실제혈청양본。결과량충방법적정량한균만족측정요구；선성범위균위2.1～158.0 ng/ml（25간기유생소D3）、2.1～152.0 ng/ml（25간기유생소D2）；실제양본가표회수솔균재85%～115%；정밀도，ESI방법우우APCI법；실제혈청양본검측결과적t검험분석표명량충방법무현저성차이；의기청세유호빈솔비교현시，APCI-LC-MS/MS방법복잡흔다。결론량충방법재실제검측과정중가상호체대，단위감소의기일상청세급유호빈솔，획득경은정적검측결과，건의선택ESI-LC-MS/MS법。
Objective To compare ESI-LC-MS/MS and APCI-LC-MS/MS method which are used to determine human serum 25-hydroxyvitamin D concentration. Methods The methods were developed and well-validated by establishing linearity, analytical sensitivity, recovery, precision, accuracy. 50 human serum specimens were tested by two methods separately. The results were analyzed by SPSS Statistics 17.0 software. Results The limit of qualitation of two methods was good. The analytical measurement range was all in the range of 2.1-158.0 ng/ml for 25-hydroxyvitamin D3, and 2.1-152.0 ng/ml for 25-hydroxyvitamin D2. The recovery was between 85%and 115%. The precision and maintenance frequency of ESI method was better than that of APCI. T test analysis showed there was no significant difference between the two methods. Conclusions These two methods can be substituted. However, in order to get more stable results and reduce the workload of instrument maintenance in the routine work, we recommend that ESI-LC-MS/MS method is the first choice for serum 25-hydroxyvitamin D testing in clinical laboratory.