CAJ | 학술논문

目的：初步探究姜黄素类似物Sunwsz08诱导人非小细胞肺癌（A549）细胞自噬现象和抑制增殖的作用。方法选用不同浓度的姜黄素类似物Sunwsz08作用于体外培养的A549细胞中，于倒置显微镜下观察细胞形态；吖啶橙、PI染色药物作用后在荧光显微镜下观察A549细胞内酸性颗粒及细胞死亡情况；MTT法检测Sunwsz08对A549细胞增殖的抑制作用；GFP-LC3自噬定位法观察细胞自噬泡。结果姜黄素类似物Sunwsz08在16μmol/L、20μmol/L及更高浓度时，A549细胞空泡增加，细胞形态明显异常，细胞死亡现象常见。Sunwsz08在8μmol/L、10μmol/L浓度时，吖啶橙染色可见明显酸性小泡；PI染色发现随着药物浓度增加及作用时间的延长，A549细胞死亡现象增加。MTT法检测示姜黄素类似物Sunwsz08对A549细胞增殖的抑制作用明显，其抑制效果随着药物浓度增加和时间的延长而增强。结论姜黄素类似物Sunwsz08可诱导A549细胞自噬并且对细胞增殖有抑制作用。
목적：초보탐구강황소유사물Sunwsz08유도인비소세포폐암（A549）세포자서현상화억제증식적작용。방법선용불동농도적강황소유사물Sunwsz08작용우체외배양적A549세포중，우도치현미경하관찰세포형태；아정등、PI염색약물작용후재형광현미경하관찰A549세포내산성과립급세포사망정황；MTT법검측Sunwsz08대A549세포증식적억제작용；GFP-LC3자서정위법관찰세포자서포。결과강황소유사물Sunwsz08재16μmol/L、20μmol/L급경고농도시，A549세포공포증가，세포형태명현이상，세포사망현상상견。Sunwsz08재8μmol/L、10μmol/L농도시，아정등염색가견명현산성소포；PI염색발현수착약물농도증가급작용시간적연장，A549세포사망현상증가。MTT법검측시강황소유사물Sunwsz08대A549세포증식적억제작용명현，기억제효과수착약물농도증가화시간적연장이증강。결론강황소유사물Sunwsz08가유도A549세포자서병차대세포증식유억제작용。
Objective To explore the ability of curcumin analog Sunwsz08 to induce autophagy in A549 cells as well as inhibit their proliferation. Methods A549 cultures were incubated with various concentrations of Sunwsz08 for different durations,and their mor-phology was examined using an inverted microscope.The cultures were stained with acridine orange and propidium iodide,and their morphology was further examined by fluorescence microscopy.In order to examine the ability of Sunwsz08 to promote autophagy,GFP-LC3 positioning was used to identify autophagic vacuoles.The MTT assay was used to assess the compound’s ability to inhibit cellular proliferation. Results At concentrations of 8 and 10 mmol/L,Sunwsz08 significantly increased autophagy in A549 cells.At concentra-tions above 16 mmol/L,the analog caused an increase in the number of cell vacuoles,and it induced abnormal cell morphology and cell death.Sunwsz08 also inhibited A549 proliferation in a dose-and time-dependent manner. Conclusion The curcumin analogue Sunwsz08 can induce autophagy and inhibit proliferation in A549 cells.