CAJ | 학술논문

目的：比较变应性鼻炎(AR)大鼠模型制作过程中鼻腔不同激发方式的效果，探索较优的激发方式。方法32只SD大鼠随机分为Ⅰ组、Ⅱ组、Ⅲ组、Ⅳ组(空白组)各8只，Ⅰ组、Ⅱ组、Ⅲ组分别采用OVA滴鼻、OVA雾化、OVA鼻腔强化激发(滴鼻+雾化)、生理盐水鼻腔强化激发的方式激发，采用症状评分及鼻黏膜HE染色镜下观察的方式，比较其制作AR大鼠模型的效果。结果Ⅰ组、Ⅱ组、Ⅲ组症状评分均数均大于5，与Ⅳ组(空白组)比较，有统计学意义(P<0.05)，Ⅲ组(鼻腔强化激发)优于Ⅰ组、Ⅱ组，有统计学意义(P<0.05)。Ⅰ组与Ⅱ组效果相当(P>0.05)。结论制作AR大鼠模型时，在低剂量佐剂，长时间基础致敏的基础上，采用鼻腔强化激发（即滴鼻+雾化吸入）的方式激发，其效果明显优于单用一种方式激发。
목적：비교변응성비염(AR)대서모형제작과정중비강불동격발방식적효과，탐색교우적격발방식。방법32지SD대서수궤분위Ⅰ조、Ⅱ조、Ⅲ조、Ⅳ조(공백조)각8지，Ⅰ조、Ⅱ조、Ⅲ조분별채용OVA적비、OVA무화、OVA비강강화격발(적비+무화)、생리염수비강강화격발적방식격발，채용증상평분급비점막HE염색경하관찰적방식，비교기제작AR대서모형적효과。결과Ⅰ조、Ⅱ조、Ⅲ조증상평분균수균대우5，여Ⅳ조(공백조)비교，유통계학의의(P<0.05)，Ⅲ조(비강강화격발)우우Ⅰ조、Ⅱ조，유통계학의의(P<0.05)。Ⅰ조여Ⅱ조효과상당(P>0.05)。결론제작AR대서모형시，재저제량좌제，장시간기출치민적기출상，채용비강강화격발（즉적비+무화흡입）적방식격발，기효과명현우우단용일충방식격발。
Objective Purpose For choose better nasal stimulate in making rat model of allergic rhinitis,we compared different method of nasal stimulate.Methods 32 rats were divided into 4 groups randomly(Ⅰ、Ⅱ、Ⅲ、Ⅳ),and received four different nasal stimulates respectively,which were OVA nasal dripping,OVA aerosol inhalation,OVA nasal strengthening stimulate(OVA nasal dripping+ OVA aerosol inhalation),normal saline nasal strengthening stimulate.The compared the different effect through symptom score and observing nasal mucosa by electron microscope.Results The symptom score were higher than 5 in groupⅠ,groupⅡand groupⅢ,which was better than group Ⅳ(P<0.05).The symptom score of group Ⅲ was higher than groupⅠand groupⅡ(P<0.05).GroupⅠand group Ⅱhad similar score(P>0.05).Conclusion When make rat model of allergic rhinitis,OVA nasal strengthening stimulate was better than just one nasal stimulate (OVA nasal dripping or OVA aerosol inhalation) based on low doses adjuvant and longer time foundation sensitization.