CAJ | 학술논문

目的：研究ACC-αRNA干扰对前列腺癌干细胞增殖的影响。方法设计并合成ACC-α基因的小干扰RNA（siRNA），转染前列腺癌干细胞系，通过Western blot检测RNA干扰对ACC-α表达的影响，并使用MTT法检测RNA干扰对细胞增殖的影响，使用荧光染色法分析前列腺癌干细胞的凋亡状况。结果 siRNA干扰96 h后可见ACC-α表达显著下调，转染96 h后前列腺癌干细胞增殖状况显著下降，通过荧光染色观察发现，siRN A干扰造成癌细胞的大量凋亡。结论 siRNA 干扰 ACC-α可以通过诱导凋亡显著的抑制前列腺癌干细胞的增殖， ACC-α是前列腺癌潜在的治疗靶点。
목적：연구ACC-αRNA간우대전렬선암간세포증식적영향。방법설계병합성ACC-α기인적소간우RNA（siRNA），전염전렬선암간세포계，통과Western blot검측RNA간우대ACC-α표체적영향，병사용MTT법검측RNA간우대세포증식적영향，사용형광염색법분석전렬선암간세포적조망상황。결과 siRNA간우96 h후가견ACC-α표체현저하조，전염96 h후전렬선암간세포증식상황현저하강，통과형광염색관찰발현，siRN A간우조성암세포적대량조망。결론 siRNA 간우 ACC-α가이통과유도조망현저적억제전렬선암간세포적증식， ACC-α시전렬선암잠재적치료파점。
Objective To investigate the role of ACC-α siRNA interference in prostate stem cells proliferation . Methods The siRNA of ACC-αand transfect prostate stem cell were designed and synthetized .The effect of siRNA on ACC-αexpression was detected by western blot .MTT assay was used to detect the prostate stem cell proliferation and the apoptosis of prostate stem cells were evaluated by fluorescent staining .Results The expression of ACC-αwas significantly lower and the propagation of prostate cancer stem cells decreased obviously after 96 h transfection . There was a large number of cancer cell apoptosis under fluorescence microscopy by siRNA interference . Conclusion siRNA interference ACC-αcan suppress prostate proliferation through inducing cell apoptosis ,which was a potential therapeutic target of prostate cancer .