检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2013年
22期
2949-2951
,共3页
载脂蛋白(a)%五核苷酸重复序列%蒙古族%脑梗死%脂蛋白(a)
載脂蛋白(a)%五覈苷痠重複序列%矇古族%腦梗死%脂蛋白(a)
재지단백(a)%오핵감산중복서렬%몽고족%뇌경사%지단백(a)
apolipoprotein(a)%pentanucleotide repeat%Mongolian%cerebral infarction%lipoprotein(a)
目的：研究载脂蛋白（a）[apo（a）]5′调控区（TTTTA）n五核苷酸重复序列（PNR）基因多态性在内蒙古地区蒙古族脑梗死患者中的分布特点。方法采用聚合酶链反应-聚丙烯酰胺凝胶电泳（PCR-PAGE）方法检测66例蒙古族健康人群及67例蒙古族脑梗死患者的apo（a）的PNR基因多态性。结果脑梗死患者血浆脂蛋白（a）[Lp（a）]、三酰甘油（TG）水平明显高于健康对照组，高密度脂蛋白-C（HDL-C）水平明显低于健康对照组；脑梗死患者组检测出（TTTTA）序列重复次数为5、8、9共3种等位基因，5/5、5/8、5/9、8/8、8/9、9/9共6种基因型；健康对照组检测出（TTTTA）序列重复次数为5、7、8、9共4种等位基因，5/8、5/5、7/9、8/8、8/9、9/9共6种基因型。两组人群均以重复次数为8和基因型8/8分布频率最高；脑梗死组与健康对照组apo（a）PNR比较发现各等位基因及基因型频率间差异无统计学意义；脑梗死组基因型8/8与8/9间的血浆Lp（a）水平比较差异无统计学意义。结论蒙古族人群apo（a）PNR基因多态性可能与脑梗死发生不存在直接的相关性。
目的：研究載脂蛋白（a）[apo（a）]5′調控區（TTTTA）n五覈苷痠重複序列（PNR）基因多態性在內矇古地區矇古族腦梗死患者中的分佈特點。方法採用聚閤酶鏈反應-聚丙烯酰胺凝膠電泳（PCR-PAGE）方法檢測66例矇古族健康人群及67例矇古族腦梗死患者的apo（a）的PNR基因多態性。結果腦梗死患者血漿脂蛋白（a）[Lp（a）]、三酰甘油（TG）水平明顯高于健康對照組，高密度脂蛋白-C（HDL-C）水平明顯低于健康對照組；腦梗死患者組檢測齣（TTTTA）序列重複次數為5、8、9共3種等位基因，5/5、5/8、5/9、8/8、8/9、9/9共6種基因型；健康對照組檢測齣（TTTTA）序列重複次數為5、7、8、9共4種等位基因，5/8、5/5、7/9、8/8、8/9、9/9共6種基因型。兩組人群均以重複次數為8和基因型8/8分佈頻率最高；腦梗死組與健康對照組apo（a）PNR比較髮現各等位基因及基因型頻率間差異無統計學意義；腦梗死組基因型8/8與8/9間的血漿Lp（a）水平比較差異無統計學意義。結論矇古族人群apo（a）PNR基因多態性可能與腦梗死髮生不存在直接的相關性。
목적：연구재지단백（a）[apo（a）]5′조공구（TTTTA）n오핵감산중복서렬（PNR）기인다태성재내몽고지구몽고족뇌경사환자중적분포특점。방법채용취합매련반응-취병희선알응효전영（PCR-PAGE）방법검측66례몽고족건강인군급67례몽고족뇌경사환자적apo（a）적PNR기인다태성。결과뇌경사환자혈장지단백（a）[Lp（a）]、삼선감유（TG）수평명현고우건강대조조，고밀도지단백-C（HDL-C）수평명현저우건강대조조；뇌경사환자조검측출（TTTTA）서렬중복차수위5、8、9공3충등위기인，5/5、5/8、5/9、8/8、8/9、9/9공6충기인형；건강대조조검측출（TTTTA）서렬중복차수위5、7、8、9공4충등위기인，5/8、5/5、7/9、8/8、8/9、9/9공6충기인형。량조인군균이중복차수위8화기인형8/8분포빈솔최고；뇌경사조여건강대조조apo（a）PNR비교발현각등위기인급기인형빈솔간차이무통계학의의；뇌경사조기인형8/8여8/9간적혈장Lp（a）수평비교차이무통계학의의。결론몽고족인군apo（a）PNR기인다태성가능여뇌경사발생불존재직접적상관성。
Objective To investigate the relationship between pentanucleotide repeat (PNR) polymorphism of apolipoprotein(a) gene and cerebral infarction in Mongolian .Methods PNR polymorphism of the apo (a) gene from 66 healthy persons and 67 patients with cerebral infarction were analyzed by polymerase chain reaction-polyacrylam-ide gel electrophoresis-silver stain(PCR-PAGE) .Results The concentrations of Lp(a) and TG in cerebral infarction patients were significantly elevated compared with normal subjects .However ,HDL-C was obviously decreased .In cerebral infarction patients ,there were 3 alleles ,which were5 ,8 and 9 repeats .Moreover ,we detected 6 apo(a) PNR genetypes ,which were 5/5 ,5/8 ,5/9 ,8/8 ,8/9 and 9/9 .In control group ,4 alleles were detected ,namely 5 ,7 ,8 and 9 . At the same time ,we found 6 genetypes ,they were 5/8 ,5/5 ,7/9 ,8/8 ,8/9 and 9/9 .Among them ,8 repeats and gene-types 8/8 had the highest distribution frequency .The frequency of apo(a) PNR alleles and genetypes had no signifi-cant difference between cerebral infarction patients and controls .Conclusion Perhaps ,apo (a) PNR polymorphisms have no direct relationship with cerebral infarction in Mongolian .
