CAJ | 학술논문

背景：国内外众多学者将丝素蛋白应用到小口径人工血管中，得到的人工血管拥有较好的抗血栓性及优秀的机械强度，动物实验也取得了满意的远期通畅率。目的：概述丝素蛋白的生物化学性质，目前在血管中常用的改性手段，以及国内外小口径人工血管中丝素蛋白的具体应用。方法：应用计算机检索中国知网、Medline数据库及ScienceDirect数据库1980年1月至2013年12月有关小口径人造血管血液相容性方面的文献，中文检索词为“人工血管，丝素蛋白”，英文检索词为“Vascular grafts， silk fibroin”。结果与结论：丝素蛋白拥有可控的机械性能，可通过醇化等处理获得不同的顺应性。同时，丝素蛋白拥有良好的生物相容性，分解产物已知、无毒，满足生物医药材料的安全需求。此外，丝素蛋白易于整合抗凝集团，效果明显可靠，且易于内皮细胞定植生长，而短期内能否形成稳定牢固的内皮层是保证血管假体拥有理想远期通畅率的关键，因此丝素蛋白是制备小口径人工血管的理想材料。但是由于制作工艺等问题，中国早期应用丝素蛋白制作的人工血管并未得到长远的发展。近年来，随着纳米材料、仿生技术、组织工程类血管、静电纺丝等技术的研究发展，以及众多学者为应对小口径血管中复杂血流动力学环境对多层血管的研究尝试，为丝素蛋白在人工血管中的应用开辟了新的空间领域。
배경：국내외음다학자장사소단백응용도소구경인공혈관중，득도적인공혈관옹유교호적항혈전성급우수적궤계강도，동물실험야취득료만의적원기통창솔。목적：개술사소단백적생물화학성질，목전재혈관중상용적개성수단，이급국내외소구경인공혈관중사소단백적구체응용。방법：응용계산궤검색중국지망、Medline수거고급ScienceDirect수거고1980년1월지2013년12월유관소구경인조혈관혈액상용성방면적문헌，중문검색사위“인공혈관，사소단백”，영문검색사위“Vascular grafts， silk fibroin”。결과여결론：사소단백옹유가공적궤계성능，가통과순화등처리획득불동적순응성。동시，사소단백옹유량호적생물상용성，분해산물이지、무독，만족생물의약재료적안전수구。차외，사소단백역우정합항응집단，효과명현가고，차역우내피세포정식생장，이단기내능부형성은정뢰고적내피층시보증혈관가체옹유이상원기통창솔적관건，인차사소단백시제비소구경인공혈관적이상재료。단시유우제작공예등문제，중국조기응용사소단백제작적인공혈관병미득도장원적발전。근년래，수착납미재료、방생기술、조직공정류혈관、정전방사등기술적연구발전，이급음다학자위응대소구경혈관중복잡혈류동역학배경대다층혈관적연구상시，위사소단백재인공혈관중적응용개벽료신적공간영역。
BACKGROUND:Many scholars have successfuly applied silk fibroin into smal-caliber vascular prostheses to achieve better anti-thrombotic role and excelent mechanical strength. In the animal experiments, an ideal long-term patency has been achieved. OBJECTIVE:To summarize the biochemical properties of silk fibroin, the currently used methods for the vessel modification, as wel as specific applications of silk fibroin in smal-caliber vascular prostheses. Methods: A computer-based search of CNKI, Medline database and ScienceDirect databases from January 1980 to December 2013 was performed for articles relevant to smal-caliber vascular prostheses with the key words of “vascular grafts, silk fibroin” in Chinese and English. RESULTS AND CONCLUSION:Silk fibroin has controlable mechanical properties and good biocompatibility. Its decomposition products is wel known and non-toxic. In addition, silk fibroin is easy to be integrated by anticoagulant group, and the effect is obvious, reliable, and easy for colonization and growth of endothelial cels, which can bring a satisfactory long-term patency. So, silk fibroin is an ideal material for the smal-caliber vascular prostheses. However, due to the production process and other issues, the application of silk fibroin in previous studies has no long-term development. Recently, the development of nano-materials, bionic technology, vascular tissue engineering, and electrospinning technology brings new chances for the application of silk fibroin in artificial vessels.