科教文汇
科教文彙
과교문회
EDUCATION SCIENCE & CULTURE MAGAZINE
2014年
12期
94-95
,共2页
盐酸多巴酚丁胺%荧光法%血红蛋白
鹽痠多巴酚丁胺%熒光法%血紅蛋白
염산다파분정알%형광법%혈홍단백
dobutamine hydrochloride%spectrofluorimetry%hemo-globin
利用盐酸多巴酚丁胺对血红蛋白模拟酶催化L-酪氨酸和H2O2体系的抑制作用,建立了酶催化荧光法测定盐酸多巴酚丁胺的新方法。研究了该猝灭反应的最佳实验条件,其猝灭程度与盐酸多巴酚丁胺的浓度呈线性关系。测定的线性范围为5.92×10-7～8.88×10-6mol/L,检出限为1.43×10-9mol/L。对浓度为5.92×10-7mol/L的盐酸多巴酚丁胺溶液进行11次平行测定,其相对标准偏差为3.2%。该方法用于药物中盐酸多巴酚丁胺含量的测定,结果满意。
利用鹽痠多巴酚丁胺對血紅蛋白模擬酶催化L-酪氨痠和H2O2體繫的抑製作用,建立瞭酶催化熒光法測定鹽痠多巴酚丁胺的新方法。研究瞭該猝滅反應的最佳實驗條件,其猝滅程度與鹽痠多巴酚丁胺的濃度呈線性關繫。測定的線性範圍為5.92×10-7～8.88×10-6mol/L,檢齣限為1.43×10-9mol/L。對濃度為5.92×10-7mol/L的鹽痠多巴酚丁胺溶液進行11次平行測定,其相對標準偏差為3.2%。該方法用于藥物中鹽痠多巴酚丁胺含量的測定,結果滿意。
이용염산다파분정알대혈홍단백모의매최화L-락안산화H2O2체계적억제작용,건립료매최화형광법측정염산다파분정알적신방법。연구료해졸멸반응적최가실험조건,기졸멸정도여염산다파분정알적농도정선성관계。측정적선성범위위5.92×10-7～8.88×10-6mol/L,검출한위1.43×10-9mol/L。대농도위5.92×10-7mol/L적염산다파분정알용액진행11차평행측정,기상대표준편차위3.2%。해방법용우약물중염산다파분정알함량적측정,결과만의。
A high sensitive and simple spectrofluorimetric method for the determination of dobutanmine hydrochloride based on its inhibitory effect on the hemoglobin-catalyzed reaction of H2O2 and L-tyrosine was developed. The concentration of dobutanmine hydrochloride is in linear relationship with the fluorescence quenching (ΔF) of system under the optimal experimental con-ditions. The calibration graph is linear in the range from 5.92í10-7 to 8.88í10-6 mol/L with the detection limit of 1.43í10-9 mol/L. The relative standard deviation for 11 replicate determi-nation of 5.92í10-7 mol/L dobutamine hydrochloride was 3.2%. This method can be used for the determination of dobutanmine hydrochloride in its pharmaceutical formulations and urine with satisfactory results.
