南通大学学报（医学版）
南通大學學報（醫學版）
남통대학학보（의학판）
JOURNAL OF NANTONG UNIVERSITY(MEDICAL SCIENCES)
2014年
3期
180-182
,共3页
脑梗死%奥拉西坦%细胞凋亡%神经再生%大鼠
腦梗死%奧拉西坦%細胞凋亡%神經再生%大鼠
뇌경사%오랍서탄%세포조망%신경재생%대서
ischemic stroke%oxiracetam%apoptosis%neurogenesis%rat
目的：探讨奥拉西坦(oxiracetam)对脑梗死大鼠细胞凋亡及神经再生的影响。方法：60只健康成年雄性SD大鼠随机分为假手术组、生理盐水组、奥拉西坦组(n=20/组)。大鼠右侧大脑中动脉阻塞脑缺血模型成功后，生理盐水组给予尾静脉注射无菌生理盐水1 mL，连续14 d；奥拉西坦组尾静脉注射无菌生理盐水配置的奥拉西坦1 mL，注射剂量为200 mg/kg，连续14 d；假手术组仅切开颈部肌肉，暴露颈总动脉后缝合。模型成功后同时给予大鼠腹腔注射BrdU (50 mg/kg)，bid，连续7 d以标记新生细胞。比较各组动物的神经功能评分，用TUNEL荧光标记测定细胞凋亡，BrdU、微管相关蛋白(doublecortin，DCX)免疫荧光技术标记增殖细胞及新生神经元。结果：与假手术组相比，脑梗死(生理盐水组和奥拉西坦组)大鼠的神经功能明显降低(均 P<0.05)；治疗后，奥拉西坦组的大鼠神经功能比生理盐水组明显改善，差异有统计学意义(P<0.05)。脑梗死后，大鼠缺血脑组织的细胞凋亡明显增加，但奥拉西坦组的细胞凋亡明显少于生理盐水组，而增殖细胞及新生神经元显著多于假手术组和生理盐水组(P<0.05)。结论：奥拉西坦可减少细胞凋亡、促进神经再生，从而改善脑梗死大鼠的神经功能。
目的：探討奧拉西坦(oxiracetam)對腦梗死大鼠細胞凋亡及神經再生的影響。方法：60隻健康成年雄性SD大鼠隨機分為假手術組、生理鹽水組、奧拉西坦組(n=20/組)。大鼠右側大腦中動脈阻塞腦缺血模型成功後，生理鹽水組給予尾靜脈註射無菌生理鹽水1 mL，連續14 d；奧拉西坦組尾靜脈註射無菌生理鹽水配置的奧拉西坦1 mL，註射劑量為200 mg/kg，連續14 d；假手術組僅切開頸部肌肉，暴露頸總動脈後縫閤。模型成功後同時給予大鼠腹腔註射BrdU (50 mg/kg)，bid，連續7 d以標記新生細胞。比較各組動物的神經功能評分，用TUNEL熒光標記測定細胞凋亡，BrdU、微管相關蛋白(doublecortin，DCX)免疫熒光技術標記增殖細胞及新生神經元。結果：與假手術組相比，腦梗死(生理鹽水組和奧拉西坦組)大鼠的神經功能明顯降低(均 P<0.05)；治療後，奧拉西坦組的大鼠神經功能比生理鹽水組明顯改善，差異有統計學意義(P<0.05)。腦梗死後，大鼠缺血腦組織的細胞凋亡明顯增加，但奧拉西坦組的細胞凋亡明顯少于生理鹽水組，而增殖細胞及新生神經元顯著多于假手術組和生理鹽水組(P<0.05)。結論：奧拉西坦可減少細胞凋亡、促進神經再生，從而改善腦梗死大鼠的神經功能。
목적：탐토오랍서탄(oxiracetam)대뇌경사대서세포조망급신경재생적영향。방법：60지건강성년웅성SD대서수궤분위가수술조、생리염수조、오랍서탄조(n=20/조)。대서우측대뇌중동맥조새뇌결혈모형성공후，생리염수조급여미정맥주사무균생리염수1 mL，련속14 d；오랍서탄조미정맥주사무균생리염수배치적오랍서탄1 mL，주사제량위200 mg/kg，련속14 d；가수술조부절개경부기육，폭로경총동맥후봉합。모형성공후동시급여대서복강주사BrdU (50 mg/kg)，bid，련속7 d이표기신생세포。비교각조동물적신경공능평분，용TUNEL형광표기측정세포조망，BrdU、미관상관단백(doublecortin，DCX)면역형광기술표기증식세포급신생신경원。결과：여가수술조상비，뇌경사(생리염수조화오랍서탄조)대서적신경공능명현강저(균 P<0.05)；치료후，오랍서탄조적대서신경공능비생리염수조명현개선，차이유통계학의의(P<0.05)。뇌경사후，대서결혈뇌조직적세포조망명현증가，단오랍서탄조적세포조망명현소우생리염수조，이증식세포급신생신경원현저다우가수술조화생리염수조(P<0.05)。결론：오랍서탄가감소세포조망、촉진신경재생，종이개선뇌경사대서적신경공능。
Objective: To study the effects of oxiracetam on apoptosis and neurogenesis in rat ischemic stroke model. Methods: 60 adult male Sprague Dawley rats were randomly separated into three groups, including sham operation group, saline group, oxiracetam group(n=20/group). Rat right middle cerebral artery transient occlusion(MCAO) model was established. Two separate groups were administered with either oxiracetam or saline via the tail vein immediately after artery occlusion for 14 days . Neck muscles and carotid artery were in sham operation group exposed . And BrdU ( 50 mg/kg ) was performed intraperitoneal injection to all rats twice a day for 7 days to label stem cells. The neural function was compared among the three groups. TUNEL immunofluorescence was used to detect apoptosis , BrdU and DCX immunofluorescence were used to observe neurogenesis in ischemic brain. Results: After MCAO, the neural function in saline group and oxiracetam group obviously decreased(P<0.05) as compared with sham operation group. But the function in oxiracetam group was better than that in saline group after treatment ( P<0 . 05 ) . The number of BrdU and DCX positive cell was significantly higher than those in sham operation group and saline group(P<0.05 respectively), while the TUNEL positive cell in oxiracetam group was less than that in saline group. Conclusion: Oxiracetam may promote neuronal function via reducing apoptosis and stimulating neurogenesis.
