CAJ | 학술논문

目的：观察商陆皂苷甲(EsA)对白细胞介素1β（IL-1β）诱导的大鼠肾小球系膜细胞(rGMC)增殖及Caspase-3表达的影响，探讨EsA治疗狼疮性肾炎等系膜细胞增殖性肾小球肾炎的可能机制。方法 MTT法检测不同浓度的EsA对rGMC增殖的影响；蛋白质印迹法检测Caspase-3的表达，并成像分析。结果观察剂量中EsA对rGMC没有明显的细胞毒作用，EsA作用rGMC 48 h后抑制细胞增殖的有效浓度为2.5～5.0 mg/L(P＜0.05或P＜0.01)；IL-1β促进了rGMC的Caspase-3的表达(P＜0.01)，与IL-1β组比较，EsA+IL-1β组对rGMC的Caspase-3的表达差异无统计学意义（P＞0.05）。结论在体外EsA(2.5～5 mg/L)可显著抑制rGMC的增殖，GMC可能是EsA的作用靶细胞；但其促凋亡作用不明显。
목적：관찰상륙조감갑(EsA)대백세포개소1β（IL-1β）유도적대서신소구계막세포(rGMC)증식급Caspase-3표체적영향，탐토EsA치료랑창성신염등계막세포증식성신소구신염적가능궤제。방법 MTT법검측불동농도적EsA대rGMC증식적영향；단백질인적법검측Caspase-3적표체，병성상분석。결과관찰제량중EsA대rGMC몰유명현적세포독작용，EsA작용rGMC 48 h후억제세포증식적유효농도위2.5～5.0 mg/L(P＜0.05혹P＜0.01)；IL-1β촉진료rGMC적Caspase-3적표체(P＜0.01)，여IL-1β조비교，EsA+IL-1β조대rGMC적Caspase-3적표체차이무통계학의의（P＞0.05）。결론재체외EsA(2.5～5 mg/L)가현저억제rGMC적증식，GMC가능시EsA적작용파세포；단기촉조망작용불명현。
Objective To observe the effects of Esculemoside A(EsA)on the proliferation and expression of Caspase-3 of rats glomerular mesangial cell(GMC)and to explore the mechanism by which EsA on effects Lupus nephritis(LN)and other GMC proliferation diseases.Methods rGMC was cultured in vitro, The cell growth were detected by MTT assay;The expression of Caspase-3 were measured by Western-Blot. Results The observed dose EsA has not apparent cytotoxicity effect on rGMC. After 48 h,inhibition proliferation concentration of EsA on rGMC was 2.5-5.0 mg/L;IL-1βpromoted the expression of Caspase-3 of rGMC(P<0.01).But there was no signiifcantly difference between IL-1βgroup and IL-1β+EsA group.Conclusion EsA(2.5-5 mg/L) can signiifcantly inhibit the proliferation of rGMC in vitro.The GMC is one of the mainly target cell. But the effect of promote apoptosis is not obvious.