湖北农业科学
湖北農業科學
호북농업과학
2014年
10期
2365-2367,2375
,共4页
紫花苜蓿%Zn2+胁迫%生理特性
紫花苜蓿%Zn2+脅迫%生理特性
자화목숙%Zn2+협박%생리특성
alfalfa(Medikageo staival L.)%zinc stress%biochemical characteristics
以紫花苜蓿品种敖汉、金皇后、三得利和隆西为材料,采用土培试验方法,研究了Zn2+胁迫对4种紫花苜蓿叶片酶促防御系统的保护酶SOD、CAT 和APX活性和H2O2、MDA含量及生物量的影响。结果表明,随着Zn2+胁迫程度的加剧,4种紫花苜蓿叶片H2O2、MDA含量均呈增加趋势,这说明它们分别遭受了Zn2+所造成的氧化胁迫,且Zn2+胁迫程度越大其遭受的氧化胁迫也越大。不同浓度Zn2+胁迫下,4种苜蓿叶片抗氧化酶活性与生物量积累相比较,敖汉的SOD、CAT、APX活性和生物量含量显著高于其他3种苜蓿,且差异显著。综合评价表明,敖汉的抗氧化能力最强,其次为金皇后和三得利,隆西的抗氧化能力最差。
以紫花苜蓿品種敖漢、金皇後、三得利和隆西為材料,採用土培試驗方法,研究瞭Zn2+脅迫對4種紫花苜蓿葉片酶促防禦繫統的保護酶SOD、CAT 和APX活性和H2O2、MDA含量及生物量的影響。結果錶明,隨著Zn2+脅迫程度的加劇,4種紫花苜蓿葉片H2O2、MDA含量均呈增加趨勢,這說明它們分彆遭受瞭Zn2+所造成的氧化脅迫,且Zn2+脅迫程度越大其遭受的氧化脅迫也越大。不同濃度Zn2+脅迫下,4種苜蓿葉片抗氧化酶活性與生物量積纍相比較,敖漢的SOD、CAT、APX活性和生物量含量顯著高于其他3種苜蓿,且差異顯著。綜閤評價錶明,敖漢的抗氧化能力最彊,其次為金皇後和三得利,隆西的抗氧化能力最差。
이자화목숙품충오한、금황후、삼득리화륭서위재료,채용토배시험방법,연구료Zn2+협박대4충자화목숙협편매촉방어계통적보호매SOD、CAT 화APX활성화H2O2、MDA함량급생물량적영향。결과표명,수착Zn2+협박정도적가극,4충자화목숙협편H2O2、MDA함량균정증가추세,저설명타문분별조수료Zn2+소조성적양화협박,차Zn2+협박정도월대기조수적양화협박야월대。불동농도Zn2+협박하,4충목숙협편항양화매활성여생물량적루상비교,오한적SOD、CAT、APX활성화생물량함량현저고우기타3충목숙,차차이현저。종합평개표명,오한적항양화능력최강,기차위금황후화삼득리,륭서적항양화능력최차。
Effects of Zn2+ stress on four alfalfa (Medikageo staival L.) including Aohan,Golden Empress,Sanditi,and Longxi were studied with soil culture. The indexes including the protective enzymes activities of SOD,CAT,APX,the con tent of peroxidation and MDA,and biomass contents were determined. The results showed that the MDA and H2O2 contents in the leaves of four alfalfa species increased with the increasing levels of Zn2+ stress. All alfalfa suffered oxidative damage under different levels of Zn2+ stress. The oxidative damage became more serious with increasing levels of Zn2+ stress. Under different levels of Zn2+ stress,Aohan had the highest activities of SOD,CAT,APX and biomass contents, significantly different with other species.The order of antioxidant capacity of the four alfalfa species was Aohan,Golden Empress,Sanditi and Longxi.