湖北农业科学
湖北農業科學
호북농업과학
2014年
10期
2344-2346,2351
,共4页
番茄%浸胚法%外源DNA%生理指标
番茄%浸胚法%外源DNA%生理指標
번가%침배법%외원DNA%생리지표
tomato%dip embryo law%exogenous DNA%physiological indicators
通过提取大豆DNA，采用浸胚法在不同的浓度和温度下来处理番茄种子，观察后代植株一系列生理指标的变化，通过用紫外分光光度法测定后代植株叶绿素含量，用聚丙烯酰胺凝胶电泳（PAGE)对植株同工酶（SOD)进行分析，发现经处理的番茄种子，后代植株性状产生变异：叶绿素总含量与对照相比出现增加，其中DNA浓度为136.50μg/mL经4℃浸泡避光处理的番茄种子总叶绿素含量变化最大，比对照增加了116%；同工酶（SOD)谱带一致，没有新的谱带出现，但酶活发生了变化。
通過提取大豆DNA，採用浸胚法在不同的濃度和溫度下來處理番茄種子，觀察後代植株一繫列生理指標的變化，通過用紫外分光光度法測定後代植株葉綠素含量，用聚丙烯酰胺凝膠電泳（PAGE)對植株同工酶（SOD)進行分析，髮現經處理的番茄種子，後代植株性狀產生變異：葉綠素總含量與對照相比齣現增加，其中DNA濃度為136.50μg/mL經4℃浸泡避光處理的番茄種子總葉綠素含量變化最大，比對照增加瞭116%；同工酶（SOD)譜帶一緻，沒有新的譜帶齣現，但酶活髮生瞭變化。
통과제취대두DNA，채용침배법재불동적농도화온도하래처리번가충자，관찰후대식주일계렬생리지표적변화，통과용자외분광광도법측정후대식주협록소함량，용취병희선알응효전영（PAGE)대식주동공매（SOD)진행분석，발현경처리적번가충자，후대식주성상산생변이：협록소총함량여대조상비출현증가，기중DNA농도위136.50μg/mL경4℃침포피광처리적번가충자총협록소함량변화최대，비대조증가료116%；동공매（SOD)보대일치，몰유신적보대출현，단매활발생료변화。
Tomato seeds were treated in different concentrations of soybean DNA and temperature. Changes of a series of physiological indexes of the offsprings were observed. The content of chlorophyll of offsprings was determined by uv spectrophotometry. Polypropylene gel electrophoresis (PAGE)was used to analyze the peroxidase isozyme (SOD) of plants. Results showed that content of total chlorophyll was increased compared with contrast. When the DNA concentration was 136.50μg/mL and tomato seeds were soaked lightly at 4℃, content of total chlorophyll had the biggest changes with increase of 116%. Band of peroxidase isozyme (SOD) was consistent. There was no new band, but the activity of enzyme was changed.
