湖北农业科学
湖北農業科學
호북농업과학
2014年
10期
2312-2315
,共4页
甘彩霞%袁伟玲%王晴芳%崔磊%梅时勇
甘綵霞%袁偉玲%王晴芳%崔磊%梅時勇
감채하%원위령%왕청방%최뢰%매시용
萝卜黑斑病菌(Alternaria sp.)%JZHB7菌株%生物学特性%产孢量%培养基
蘿蔔黑斑病菌(Alternaria sp.)%JZHB7菌株%生物學特性%產孢量%培養基
라복흑반병균(Alternaria sp.)%JZHB7균주%생물학특성%산포량%배양기
radish black spot disease (Alternaria sp.)%JZHB7 Alternaria%biological characteristics%sporulation quantity%medium
从12个萝卜黑斑病菌（Alternaria sp.)菌株里筛选产孢最多的菌株，从8种培养基（PDA培养基、PSA培养基、牛肉膏蛋白胨培养基、YPD培养基、面粉培养基、豆芽培养基、玉米培养基、杏仁培养基)中筛选其最适合的培养基，研究温度、光照、培养时间对其的影响。结果表明，在12个菌株中JZHB7菌株产孢最多；豆芽培养基为JZHB7产孢的最佳培养基；JZHB7孢子15℃时萌发率高，0℃时萌发率低；JZHB7培养4~7d菌丝生长较快，10~13d生长较慢；日光灯照射培养2h产孢量多，照射培养24h产孢量少。
從12箇蘿蔔黑斑病菌（Alternaria sp.)菌株裏篩選產孢最多的菌株，從8種培養基（PDA培養基、PSA培養基、牛肉膏蛋白胨培養基、YPD培養基、麵粉培養基、豆芽培養基、玉米培養基、杏仁培養基)中篩選其最適閤的培養基，研究溫度、光照、培養時間對其的影響。結果錶明，在12箇菌株中JZHB7菌株產孢最多；豆芽培養基為JZHB7產孢的最佳培養基；JZHB7孢子15℃時萌髮率高，0℃時萌髮率低；JZHB7培養4~7d菌絲生長較快，10~13d生長較慢；日光燈照射培養2h產孢量多，照射培養24h產孢量少。
종12개라복흑반병균（Alternaria sp.)균주리사선산포최다적균주，종8충배양기（PDA배양기、PSA배양기、우육고단백동배양기、YPD배양기、면분배양기、두아배양기、옥미배양기、행인배양기)중사선기최괄합적배양기，연구온도、광조、배양시간대기적영향。결과표명，재12개균주중JZHB7균주산포최다；두아배양기위JZHB7산포적최가배양기；JZHB7포자15℃시맹발솔고，0℃시맹발솔저；JZHB7배양4~7d균사생장교쾌，10~13d생장교만；일광등조사배양2h산포량다，조사배양24h산포량소。
The bacterial strain with the largest sporulation quantity was chosen from the 12 Alternaria sp. bacterial strains and the optimal medium was selected from 8 media including PDA medium, PSA medium, beef extract peptone medium, YPD medium, flour medium, bean sprouts medium, corn medium,almond medium. The influence of temperature, light, treatment time on sporulation of Alternaria sp. were studied. The results showed that JZHB7 Alternaria had the largest sporulation quantity. Bean sprouts medium was the best medium among the eight kinds of media,JZHB7 Alternaria spores germination reached the maximum at 15℃ and the minimum at 0℃. JZHB7 Alternaria strains grew fast from 4 d to 7 d and slow from the 10 d to 13 d. Sporulation quantity of JZHB7 Alternaria increased in 2 h and decreased in 24 h.
