CAJ | 학술논문

目的：观察兔胫腓骨骨缺损模型脑室内注射瘦素蛋白（leptin）对骨折愈合速度的影响。方法建立新西兰兔右侧胫腓骨外侧骨缺损模型，leptin组经兔枕骨大孔向小脑延髓池脑脊液中注射重组兔leptin，对照组以相同方法注射等剂量生理盐水。于术后1、3、7、14、21 d记录兔体质量变化，并通过CT动态记录骨缺损区域骨痂骨密度（BMD）的变化。于术后21 d，所有动物均处死后取右侧胫腓骨骨缺损区域，以荧光原位杂交技术(FISH)检测骨折区域骨形成蛋白（BMP）-2的表达情况。结果2组动物造模后，体质量先下降后（14 d）上升，BMD一直呈上升趋势；术后1、3 d，2组体质量及BMD差异均无统计学意义，术后7、14、21 d，leptin组的体质量低于对照组，BMD高于对照组（均P＜0.05）。术后21 d，骨缺损区域BMP-2表达阳性细胞数目高于对照组(个：120.87±29.14 vs 97.65±20.97, t=6.79, P＜0.001)。结论经枕骨大孔向小脑延髓池内中注射leptin可加速兔四肢骨缺损的愈合。
목적：관찰토경비골골결손모형뇌실내주사수소단백（leptin）대골절유합속도적영향。방법건립신서란토우측경비골외측골결손모형，leptin조경토침골대공향소뇌연수지뇌척액중주사중조토leptin，대조조이상동방법주사등제량생리염수。우술후1、3、7、14、21 d기록토체질량변화，병통과CT동태기록골결손구역골가골밀도（BMD）적변화。우술후21 d，소유동물균처사후취우측경비골골결손구역，이형광원위잡교기술(FISH)검측골절구역골형성단백（BMP）-2적표체정황。결과2조동물조모후，체질량선하강후（14 d）상승，BMD일직정상승추세；술후1、3 d，2조체질량급BMD차이균무통계학의의，술후7、14、21 d，leptin조적체질량저우대조조，BMD고우대조조（균P＜0.05）。술후21 d，골결손구역BMP-2표체양성세포수목고우대조조(개：120.87±29.14 vs 97.65±20.97, t=6.79, P＜0.001)。결론경침골대공향소뇌연수지내중주사leptin가가속토사지골결손적유합。
Objective To investigate the enhanced osteogenesis during bone fracture healing after intracerebroven-tricular (ICV) leptin injection, using rabbit model with created segmental bone defect in right tibial. Method Segmental critical-sized defects were created at the right tibial bone of skeletally mature New Zealand white rabbits. In experiment group (leptin group), recombinant rabbit leptin was injected into cerebellomedullary cistern through foramen magnum. While in control group, normal saline was injected in the same way. Bone Mineral Density (BMD) was evaluated by qCT at 1st, 3rd, 7th, 14th and the 21st days. At the 21st days, all rabbits were euthanized to collect the right tibia for histomorphology, to ex-amine the BMP-2 expression in the bone callus by Fluorescence in situ hybridization (FISH). Result In the leptin group, body weight declined more obviously than control group then it start to arise at the 14th day;qCT showed significant higher BMD in leptin group than in control group at the 7th day;FISH showed a higher BMP-2 expression in leptin group than in control group. Conclusion Cerebellomedullary leptin injection through foramen magnum could accelerate limb fracture healing in rabbit model with right tibial bone defection.