天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
6期
547-550
,共4页
脊髓损伤%运动疗法%脑源性神经营养因子%受体,trkB%免疫组织化学%印迹法,蛋白质%运动训练
脊髓損傷%運動療法%腦源性神經營養因子%受體,trkB%免疫組織化學%印跡法,蛋白質%運動訓練
척수손상%운동요법%뇌원성신경영양인자%수체,trkB%면역조직화학%인적법,단백질%운동훈련
spinal cord injuries%exercise therapy%brain-derived neurotrophic factor%receptor,trkB%immunohisto-chemistry%blotting,Western%exercise training
目的：研究运动训练对脊髓损伤（SCI）大鼠脊髓内脑源性神经营养因子（BDNF）及其酪氨酸激酶受体B（TrkB）表达的影响。方法24只SD大鼠随机均分为假手术组、损伤对照组和运动训练组。采用通用型脊髓打击器建立T10 SCI大鼠模型。运动训练组于损伤后1周起对大鼠进行4周运动训练，假手术组和损伤对照组不进行运动训练。采用BBB评分观察损伤前及损伤后第1~5周大鼠后肢运动功能的变化。运动训练结束后取大鼠T12～L1节段脊髓，免疫组化结合图像平均光密度分析观察脊髓组织BDNF和TrkB的表达及分布，Western blot检测脊髓内BDNF和TrkB蛋白含量。结果损伤前，3组大鼠BBB评分为21.00分。损伤后，损伤对照组及运动训练组BBB评分均低于假手术组（均P＜0.05）。损伤3周后运动训练组BBB评分高于损伤对照组（P＜0.05）。BDNF、TrkB免疫反应阳性产物均多分布于脊髓前角、脊髓后角及中央管周围；运动训练组BDNF、TrkB阳性染色颗粒均增多，平均光密度值均高于假手术组和损伤对照组（均P＜0.05）。运动训练组大鼠脊髓内BDNF及TrkB的表达高于假手术组和损伤对照组。结论运动训练能诱导SCI大鼠脊髓内BDNF及其受体TrkB表达，促进其运动功能恢复。
目的：研究運動訓練對脊髓損傷（SCI）大鼠脊髓內腦源性神經營養因子（BDNF）及其酪氨痠激酶受體B（TrkB）錶達的影響。方法24隻SD大鼠隨機均分為假手術組、損傷對照組和運動訓練組。採用通用型脊髓打擊器建立T10 SCI大鼠模型。運動訓練組于損傷後1週起對大鼠進行4週運動訓練，假手術組和損傷對照組不進行運動訓練。採用BBB評分觀察損傷前及損傷後第1~5週大鼠後肢運動功能的變化。運動訓練結束後取大鼠T12～L1節段脊髓，免疫組化結閤圖像平均光密度分析觀察脊髓組織BDNF和TrkB的錶達及分佈，Western blot檢測脊髓內BDNF和TrkB蛋白含量。結果損傷前，3組大鼠BBB評分為21.00分。損傷後，損傷對照組及運動訓練組BBB評分均低于假手術組（均P＜0.05）。損傷3週後運動訓練組BBB評分高于損傷對照組（P＜0.05）。BDNF、TrkB免疫反應暘性產物均多分佈于脊髓前角、脊髓後角及中央管週圍；運動訓練組BDNF、TrkB暘性染色顆粒均增多，平均光密度值均高于假手術組和損傷對照組（均P＜0.05）。運動訓練組大鼠脊髓內BDNF及TrkB的錶達高于假手術組和損傷對照組。結論運動訓練能誘導SCI大鼠脊髓內BDNF及其受體TrkB錶達，促進其運動功能恢複。
목적：연구운동훈련대척수손상（SCI）대서척수내뇌원성신경영양인자（BDNF）급기락안산격매수체B（TrkB）표체적영향。방법24지SD대서수궤균분위가수술조、손상대조조화운동훈련조。채용통용형척수타격기건립T10 SCI대서모형。운동훈련조우손상후1주기대대서진행4주운동훈련，가수술조화손상대조조불진행운동훈련。채용BBB평분관찰손상전급손상후제1~5주대서후지운동공능적변화。운동훈련결속후취대서T12～L1절단척수，면역조화결합도상평균광밀도분석관찰척수조직BDNF화TrkB적표체급분포，Western blot검측척수내BDNF화TrkB단백함량。결과손상전，3조대서BBB평분위21.00분。손상후，손상대조조급운동훈련조BBB평분균저우가수술조（균P＜0.05）。손상3주후운동훈련조BBB평분고우손상대조조（P＜0.05）。BDNF、TrkB면역반응양성산물균다분포우척수전각、척수후각급중앙관주위；운동훈련조BDNF、TrkB양성염색과립균증다，평균광밀도치균고우가수술조화손상대조조（균P＜0.05）。운동훈련조대서척수내BDNF급TrkB적표체고우가수술조화손상대조조。결론운동훈련능유도SCI대서척수내BDNF급기수체TrkB표체，촉진기운동공능회복。
Objective To investigate the effects of exercise on expressions of brain-derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB) in spinal cord of spinal cord injury (SCI) rats. Methods Spinal cord injury models were produced by universal spinal cord impact system. Twenty-four Sprague-Dawley rats were randomly divided into 3 groups, exercise group (SCI-induction and exercises, n=8), control group (SCI-induction without exercises,n=8) and sham-operation group (no operation, without SCI nor exercises, n=8). Exercise training began from the 7th day after injury for 4 weeks. The locomotor function was assessed by Basso-Beattic-Bresnahan (BBB) scale before injury and at the 1st, 2nd, 3rd, 4th and 5th week post injury. The expressions level of BDNF and TrkB in spinal cords were detected by immunohistochemis-try and Western blot. Results Since the 3rd week after injury (after 2 weeks of exercise training), BBB scores in exercise group were higher than that in control group (P < 0.05). In immunohistochemistry test, positive immunologic reaction to BDNF protein was mainly located in anterior horn of spinal cord, and the expression level in exercise group was significantly higher than that in sham-operation group and control group (P<0.05). In immunohistochemistry test, positive immunologic reaction to TrkB protein was located in anterior horn and dorsal horn and central canal of spinal cord, and the expression lev-el in exercise group was significantly higher than that in sham-operation group and control group (P<0.05). Expression of BDNF and TrkB in exercise group rats after exercise for 4 weeks were significantly increased, compared with that in control group. Conclusion Exercise training may effectively induce BDNF and TrkB expression in spinal cords and promote the re-covery of locomotor function of SCI rats.
