CAJ | 학술논문

枣实蝇（ Carpomya vesuviana Costa）是一种新入侵检疫性有害生物。应用PCR-RFLP技术，快速区分鉴定了枣实蝇与我国口岸截获频率较高的5种检疫性实蝇。研究表明：设计出的2对引物对6种供试实蝇线粒体DNA （ mtDNA）进行PCR扩增，其扩增片断大小分别为350 bp和450 bp。 PCR扩增产物用2种限制性内切酶Dra I和MseI进行酶切，根据不同的酶切位点准确区分了6种供试的实蝇。此方法可用于口岸截获实蝇的快速检疫鉴定。
조실승（ Carpomya vesuviana Costa）시일충신입침검역성유해생물。응용PCR-RFLP기술，쾌속구분감정료조실승여아국구안절획빈솔교고적5충검역성실승。연구표명：설계출적2대인물대6충공시실승선립체DNA （ mtDNA）진행PCR확증，기확증편단대소분별위350 bp화450 bp。 PCR확증산물용2충한제성내절매Dra I화MseI진행매절，근거불동적매절위점준학구분료6충공시적실승。차방법가용우구안절획실승적쾌속검역감정。
Carpomya vesuviana Costa has been a new quarantine pest in China. In this paper, the method of rapid identi-fication of C. vesuviana Costa and 5 relative species of fruit flies was studied based on PCR-RFLP of the mitochondrial DNA( mtDNA) . The results showed that DNAs, which were estimated about 350 bp and 450 bp respectively, were ob-tained by means of PCR with two pairs of primers designed from mtDNA of target pests.The PCR-products could be reg-istered by restriction enzymes ( Dra I and Mse I) . All the 6 species could be discriminated according to the banding pat-terns of the restriction enzymes. It is suggested that the method is reliable for quick discrimination of fruit flies.