CAJ | 학술논문

采用菌丝干重法、半微量凯氏定氮法和磺胺比色法分别测定了HD19、ZT13和D13个树粉孢属杜鹃花菌根真菌菌株在铵态氮、硝态氮和有机氮培养基中的生物量、含氮量和硝酸还原酶活性。结果表明：3个菌株在3种氮源培养基中生长良好，而且无机氮培养基中菌株生物量和氮吸收量均显著高于有机氮培养基；其中菌株HD19在硝态氮培养基中氮吸收量显著高于铵态氮，而菌株ZT13和D1在铵态氮培养基中氮吸收量较高；菌株在硝态氮培养基中硝酸还原酶活性均较高，说明树粉孢属杜鹃花菌根真菌不仅具有较好的利用硝态氮的能力，还可以吸收利用其他形态的氮源，外源硝态氮可以诱导该类真菌硝酸还原酶活性的提高。
채용균사간중법、반미량개씨정담법화광알비색법분별측정료HD19、ZT13화D13개수분포속두견화균근진균균주재안태담、초태담화유궤담배양기중적생물량、함담량화초산환원매활성。결과표명：3개균주재3충담원배양기중생장량호，이차무궤담배양기중균주생물량화담흡수량균현저고우유궤담배양기；기중균주HD19재초태담배양기중담흡수량현저고우안태담，이균주ZT13화D1재안태담배양기중담흡수량교고；균주재초태담배양기중초산환원매활성균교고，설명수분포속두견화균근진균불부구유교호적이용초태담적능력，환가이흡수이용기타형태적담원，외원초태담가이유도해류진균초산환원매활성적제고。
In order to analyse the effects of nitrogen on the nitrogen uptake and nitrate reductase activities of ericoid my-corrhizal fungus, this study determined the mycelial biomass, nitrogen uptake and nitrate reductase activities of three strains of Oidiodendron sp.cultured on ammonium, nitrate and organic nitrogen medium respectively. The results showed that the three strains grew well in different nitrogen source medium, and the strain biomass and nitrogen uptake in inor-ganic nitrogen medium were significantly higher than that in organic nitrogen medium. The nitrogen uptake by HD19 in nitrate nitrogen medium was significantly greater than that in ammonium nitrogen medium, while the strain ZT13 and D1 had the opposite result. Nitrate reductase activities of the two strains were higher in nitrate nitrogen medium than other nitrogen source. This illustrated that the ericoid mycorrhizal fungi (Oidiodendron sp.) had the ability to utilize different nitrogen source. In all these nitrogen, the nitrate was a good one for ERM fungi growth,and it can also induce nitrate re-ductase activities of the ERM fungi.